HUMAN SPERM-CERVICAL MUCUS INTERACTION AND THE ABILITY OF SPERMATOZOA TO FUSE WITH ZONA-FREE HAMSTER OOCYTES

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dc.contributor.authorBARROS, C
dc.contributor.authorJEDLICKI, A
dc.contributor.authorFUENZALIDA, I
dc.contributor.authorHERRERA, E
dc.contributor.authorARGUELLO, B
dc.contributor.authorVIGIL, P
dc.contributor.authorVILLASECA, P
dc.contributor.authorLEONTIC, E
dc.date.accessioned2025-01-23T19:24:56Z
dc.date.available2025-01-23T19:24:56Z
dc.date.issued1988
dc.description.abstractSamples of semen and cervical mucus were provided by 18 couples. Cervical mucus was obtained for each day possible and stored at 4.degree.C until all the samples were collected. Flat capillary tubes were loaded with the mucous samples and spermatozoa from the husband''s semen sample were allowed to migrate through the cervical mucus (3 cm column) into culture medium. The spermatozoa recovered after migration through cervical mucus were assayed in vitro with zona-free hamster oocytes. Control experiments were carried out using spermatozoa from the same semen sample but prepared by the swimming-up technique. Altogether, 557 eggs in the control group and 1236 eggs in the experimental group were analysed, and the results demonstrated that the % of sperm penetration, the mean number of sperm decondensations per penetrated egg and the mean number of spermatozoa adhering per egg all had higher values (P < 0.05) for the control samples than for the experimental samples. We suggest that cervical mucus modifies human spermatozoa, as measured by their interaction with zona-free hamster oocytes.
dc.fuente.origenWOS
dc.identifier.issn0022-4251
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/99419
dc.identifier.wosidWOS:A1988M595300008
dc.issue.numero2
dc.language.isoen
dc.pagina.final484
dc.pagina.inicio477
dc.revistaJournal of reproduction and fertility
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleHUMAN SPERM-CERVICAL MUCUS INTERACTION AND THE ABILITY OF SPERMATOZOA TO FUSE WITH ZONA-FREE HAMSTER OOCYTES
dc.typeartículo
dc.volumen82
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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