Serine-Arginine Protein Kinase SRPK2 Modulates the Assembly of the Active Zone Scaffolding Protein CAST1/ERC2

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dc.contributor.authorArancibia, Duxan
dc.contributor.authorLira, Matias
dc.contributor.authorCruz, Yocelin
dc.contributor.authorBarrera, Daniela P.
dc.contributor.authorMontenegro-Venegas, Carolina
dc.contributor.authorGodoy, Juan A.
dc.contributor.authorGarner, Craig C.
dc.contributor.authorInestrosa, Nibaldo C.
dc.contributor.authorGundelfinger, Eckart D.
dc.contributor.authorZamorano, Pedro
dc.contributor.authorTorres, Viviana, I
dc.date.accessioned2025-01-23T21:08:59Z
dc.date.available2025-01-23T21:08:59Z
dc.date.issued2019
dc.description.abstractNeurons release neurotransmitters at a specialized region of the presynaptic membrane, the active zone (AZ), where a complex meshwork of proteins organizes the release apparatus. The formation of this proteinaceous cytomatrix at the AZ (CAZ) depends on precise homo- and hetero-oligomerizations of distinct CAZ proteins. The CAZ protein CAST1/ERC2 contains four coiled-coil (CC) domains that interact with other CAZ proteins, but also promote self-assembly, which is an essential step for its integration during AZ formation. The self-assembly and synaptic recruitment of the Drosophila protein Bruchpilot (BRP), a partial homolog of CAST1/ERC2, is modulated by the serine-arginine protein kinase (SRPK79D). Here, we demonstrate that overexpression of the vertebrate SRPK2 regulates the self-assembly of CAST1/ERC2 in HEK293T, SH-SY5Y and HT-22 cells and the CC1 and CC4 domains are involved in this process. Moreover, the isoform SRPK2 forms a complex with CAST1/ERC2 when co-expressed in HEK293T and SH-SY5Y cells. More importantly, SRPK2 is present in brain synaptic fractions and synapses, suggesting that this protein kinase might control the level of self-aggregation of CAST1/ERC2 in synapses, and thereby modulate presynaptic assembly.
dc.fuente.origenWOS
dc.identifier.doi10.3390/cells8111333
dc.identifier.eissn2073-4409
dc.identifier.urihttps://doi.org/10.3390/cells8111333
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/100785
dc.identifier.wosidWOS:000502266700031
dc.issue.numero11
dc.language.isoen
dc.revistaCells
dc.rightsacceso restringido
dc.subjectactive zone
dc.subjectCAST1/ERC2
dc.subjectcoiled-coil
dc.subjectself-aggregation
dc.subjectSRPK
dc.subjectsynapse
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleSerine-Arginine Protein Kinase SRPK2 Modulates the Assembly of the Active Zone Scaffolding Protein CAST1/ERC2
dc.typeartículo
dc.volumen8
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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