bFGF induces an earlier expression of nephrogenic proteins after ischemic acute renal failure

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dc.contributor.authorVillanueva, Sandra
dc.contributor.authorCespedes, Carlos
dc.contributor.authorGonzalez, Alexis
dc.contributor.authorVio, Carlos P.
dc.date.accessioned2025-01-21T01:05:48Z
dc.date.available2025-01-21T01:05:48Z
dc.date.issued2006
dc.description.abstractRecovery from acute renal failure (ARF) requires the replacement of injured cells with new cells that restore tubule epithelial integrity. We described recently the expression of a wide range of nephrogenic proteins in tubular cells after ARF induced by ischemia-reperfusion (I/R) (Villanueva S, Cespedes C, and Vio CP. Am J Physiol Regul Integr Comp Physiol 290: R861-R870, 2006). These markers, namely, Vimentin, neural cell adhesion molecules (Ncam), basic fibroblast growth factor (bFGF), paired homeobox-2 (Pax-2), bone morphogene protein-7 (BMP-7), Noggin, Lim-1, Engrailed, Smad, phospho-Smad, hypoxia-induced factor-1 alpha (HIF-1 alpha), VEGF, and Tie-2, are expressed in a time frame similar to that observed in normal kidney development. bFGF participates in early kidney development as a morphogen involved in mesenchyme/epithelial transition, and it is reexpressed in the recovery phase of ARF. To test the hypothesis that bFGF can accelerate the regeneration after renal damage, we used recombinant bFGF and studied the expression pattern of the above described morphogens in ARF. Male Sprague-Dawley rats were subjected to 30 min of renal ischemic injury and were injected with bFGF 30 mu g/kg followed by reperfusion. Rats were killed and the expression of nephrogenic proteins were analyzed by immunohistochemistry and Western blot analysis. In the animals subjected to I/R treated with bFGF, we observed a 12- to 24-h earlier and more abundant reexpression of the proteins Ncam, bFGF, Pax-2, BMP-7, Noggin, Lim-1, Engrailed, VEGF, and Tie-2 than the I/R untreated rats. In addition, we observed a reduction in renal damage markers ED-1 and alpha-smooth muscle actin. These results indicate that bFGF can participate in the regeneration process and suggest that the treatment with bFGF can induce an earlier regeneration process after ischemic acute renal failure.
dc.fuente.origenWOS
dc.identifier.doi10.1152/ajpregu.00023.2006
dc.identifier.eissn1522-1490
dc.identifier.issn0363-6119
dc.identifier.urihttps://doi.org/10.1152/ajpregu.00023.2006
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/96020
dc.identifier.wosidWOS:000241768400014
dc.issue.numero6
dc.language.isoen
dc.pagina.finalR1687
dc.pagina.inicioR1677
dc.revistaAmerican journal of physiology-regulatory integrative and comparative physiology
dc.rightsacceso restringido
dc.subjectkidney
dc.subjectmorphogen
dc.subjectregeneration
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titlebFGF induces an earlier expression of nephrogenic proteins after ischemic acute renal failure
dc.typeartículo
dc.volumen291
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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