CHEMICAL MODIFICATION OF LYSYL AND CYSTEINYL RESIDUES OF YEAST RNA POLYMERASE-I

Simple item page
dc.contributor.authorBULL, P
dc.contributor.authorZALDIVAR, J
dc.contributor.authorWYNEKEN, U
dc.contributor.authorVENEGAS, A
dc.contributor.authorVALENZUELA, P
dc.date.accessioned2025-01-23T19:44:18Z
dc.date.available2025-01-23T19:44:18Z
dc.date.issued1981
dc.description.abstractThe active site of yeast RNA polymerase I was studied using pyridoxal 5''-phosphate, p-chloromercuribenzoate and 5,5''-dithiobis (2-nitrobenzoate) as modifying agents. The enzyme was rapidly inactivated by pyridoxal 5''-phosphate, by formation of a Schiff base between the aldehyde group and lysine amino groups of the enzyme, located in the largest subunit. Out of 45 SH groups, 2 are required for enzyme activity. Since they were partially protected by substrates and DNA, they may be at the active site. A hypothetical model of catalysis is proposed based on the results presented.
dc.fuente.origenWOS
dc.identifier.issn0004-0533
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/100002
dc.identifier.wosidWOS:A1981MZ04600007
dc.issue.numero2
dc.language.isoen
dc.pagina.final147
dc.pagina.inicio143
dc.revistaArchivos de biologia y medicina experimentales
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleCHEMICAL MODIFICATION OF LYSYL AND CYSTEINYL RESIDUES OF YEAST RNA POLYMERASE-I
dc.typeartículo
dc.volumen14
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
Files
Collections
Artículos de revistas