Expression of genes encoding laccase and manganese-dependent peroxidase in the fungus <i>Ceriporiopsis subvermispora</i> is mediated by an ACE1-like copper-fist transcription factor

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dc.contributor.authorMiguel Alvarez, Jose
dc.contributor.authorCanessa, Paulo
dc.contributor.authorMancilla, Rodrigo A.
dc.contributor.authorPolanco, Ruben
dc.contributor.authorSantibanez, Paulina A.
dc.contributor.authorVicuna, Rafael
dc.date.accessioned2025-01-21T01:04:55Z
dc.date.available2025-01-21T01:04:55Z
dc.date.issued2009
dc.description.abstractThe effect of copper on the expression of genes encoding the ligninolytic enzymes laccase (ICS) and manganese peroxidase (mnp) in Ceriporiopsis subvermispora was evaluated. This metal increased transcript levels of lcs, mnp1 and mnp2. This finding was not unexpected in the case of lcs, since its promoter contains a putative ACE element. Originally characterized in the yeast Saccharomyces cerevisiae, ACE is the target sequence of the ACE1 copper-responsive transcription factor in this microorganism. Analysis of the promoter regions of mnp genes revealed the presence of formerly unnoticed ACE elements. Based on the ace1 gene from Phanerochaete chrysosporium, we isolated and characterized an ACE1-like transcription factor from C. subvermispora (Cs-ACE1) through complementation of a S. cerevisiae ace1 Delta strain. Surprisingly, ACE1 factors from both basidiomycetes exhibit substantial differences, not only structurally but also in their ability to complement the aforementioned yeast strain. Specific binding of Cs-ACE1 to its cognate DNA sequence was confirmed by electrophoretic mobility-shift assays. (C) 2008 Elsevier Inc. All rights reserved.
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.fgb.2008.10.002
dc.identifier.eissn1096-0937
dc.identifier.issn1087-1845
dc.identifier.urihttps://doi.org/10.1016/j.fgb.2008.10.002
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/95739
dc.identifier.wosidWOS:000262918600011
dc.issue.numero1
dc.language.isoen
dc.pagina.final111
dc.pagina.inicio104
dc.revistaFungal genetics and biology
dc.rightsacceso restringido
dc.subjectMulticopper oxidase
dc.subjectElectrophoretic mobility-shift assays
dc.subjectTranscription factor
dc.subjectLigninolytic
dc.subjectACE
dc.subjectcopper-fist
dc.subjectLaccase
dc.subjectManganese peroxidase
dc.subjectCeriporiopsis subvermispora
dc.subjectCopper
dc.subject.ods06 Clean Water and Sanitation
dc.subject.odspa06 Agua limpia y saneamiento
dc.titleExpression of genes encoding laccase and manganese-dependent peroxidase in the fungus <i>Ceriporiopsis subvermispora</i> is mediated by an ACE1-like copper-fist transcription factor
dc.typeartículo
dc.volumen46
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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