Short-term lipopolysaccharide stimulation indeces differentiation of murine bone marrow-derived dentritic cells into tolerogenic phenotype

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dc.contributor.authorSalazar, Lorena
dc.contributor.authorAravena, Octavio
dc.contributor.authorContreras-Levicoy, Juan
dc.contributor.authorPesce, Barbara
dc.contributor.authorCatalan, Diego
dc.contributor.authorZuniga, Roberto
dc.contributor.authorIruretagoyena, Mirentxu
dc.contributor.authorKalergis, Alexis M.
dc.contributor.authorAguillon, Juan C.
dc.date.accessioned2025-01-21T01:05:31Z
dc.date.available2025-01-21T01:05:31Z
dc.date.issued2007
dc.description.abstractDendritic cells (DCs) are professional, antigen-presenting cells, which induce and regulate T cell reactivity. DCs are crucial in innate and adaptive immune responses, and are also involved in central and peripheral tolerance induction. Tolerance can be mediated by immature and semi-mature DCs expressing low levels of co-stimulator and major histocompatibility complex (MHC) molecules. The aim of this study was to investigate the ability of short-term lipopolysaccharide (LPS) stimulation to modulate the stage of differentiation of bone marrow-derived DCs. For this purpose, DCs obtained from DBA1/lacJ mice were stimulated for four (4hLPS/DCs) or 24 (24hLPS/DCs) hours with LPS, using DCs without stimulation (0hLPS/DCs) as a control. Flow cytometry analysis of 4hLPS/DCs showed intermediate CD40 and MHC class II expression, lower than that of 24hLPS/DCs (fully mature), and greater than that of 0hLPS/DCs (immature). A functional assay showed that 4hLPS/DCs displayed increased endocytotic ability compared to 24hLPS/DCs, indicating a semi-mature state. 4hLPS/DCs were greater producers of IL-10 protein and TGF beta 1 mRNA than 24hLPS/DCs and immature DCs, displaying a cytokine production pattern that is characteristic of tolerogenic DCs. An assay for antigen-presenting capacity demonstrated that 4hLPS/DCs induced secretion of IL-2 from an OTH4 T cell hybridoma, indicating a functional presenting activity. Finally, the tolerogenic phenotype of 4hLPS/DCs was demonstrated by their ability to interfere with the progression of bovine type 11 collagen (bII)-induced arthritis (CIA) when they were loaded with bCII antigen and injected into mice with established CIA. We conclude that the stimulation of murine bone marrow-derived DCs with LPS for four hours generates semi-mature DCs with tolerogenic capability.
dc.fuente.origenWOS
dc.identifier.doi10.1684/ecn.2007.0089
dc.identifier.eissn1952-4005
dc.identifier.urihttps://doi.org/10.1684/ecn.2007.0089
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/95942
dc.identifier.wosidWOS:000248713800004
dc.issue.numero2
dc.language.isoen
dc.pagina.final85
dc.pagina.inicio78
dc.revistaEuropean cytokine network
dc.rightsacceso restringido
dc.subjectdendritic cells
dc.subjecttolerance
dc.subjectlipopolysaccharide
dc.subjectautoimmnunity
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleShort-term lipopolysaccharide stimulation indeces differentiation of murine bone marrow-derived dentritic cells into tolerogenic phenotype
dc.typeartículo
dc.volumen18
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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