IS ACTIVATED SPERMATOZOON REALLY CAPACITATED

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dc.contributor.authorBARROS, C
dc.contributor.authorBERRIOS, M
dc.date.accessioned2025-01-24T00:10:31Z
dc.date.available2025-01-24T00:10:31Z
dc.date.issued1977
dc.description.abstractA culture medium (7N) consisting only of salts and devoid of any macromolecule was prepared to test its effect upon survival, activation and acrosome reaction of golden hamster spermatozoa. Incubation of spermatozoa in medium 7N allowed a temporal separation of activation and acrosome reaction. More than 70% of spermatozoa were activated after 4 h at 37.degree. C while the acrosome reaction remained very low (8%). Activated spermatozoa with intact acrosomes were incubated with zona-free hamster oocytes at 37.degree. C for 1-2 h. When these oocytes were examined by EM it was found that the spermatozoa were closely bound to the egg plasma membrane, mainly by the plasma membrane overlying the acrosome, but in no instance had the gamete membranes fused. Prolonged incubation resulted in an engulfment of the sperm by the oocyte in a phagocytic manner. An activated spermatozoon is not capacitated since it cannot fertilize oocytes.
dc.fuente.origenWOS
dc.identifier.issn0022-104X
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/102024
dc.identifier.wosidWOS:A1977DN30800007
dc.issue.numero1
dc.language.isoen
dc.pagina.final+
dc.pagina.inicio65
dc.revistaJournal of experimental zoology
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleIS ACTIVATED SPERMATOZOON REALLY CAPACITATED
dc.typeartículo
dc.volumen201
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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