Is Single-Strand Conformation Polymorphism Analysis of the Full 5′ Untranslated Region an Adequate Approach To Study Hepatitis C Virus Quasispecies Distribution?

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dc.contributor.authorVera-Otarola, Jorge
dc.contributor.authorBarria, Maria Ines
dc.contributor.authorLeon, Ursula
dc.contributor.authorCarvallo, Pilar
dc.contributor.authorSoza, Alejandro
dc.contributor.authorLopez-Lastra, Marcelo
dc.date.accessioned2025-01-21T00:08:15Z
dc.date.available2025-01-21T00:08:15Z
dc.date.issued2009
dc.description.abstractSingle-strand conformation polymorphism (SSCP) analysis is used by many laboratories to study the quasispecies distribution of the hepatitis C virus (HCV). Here we question the validity of this experimental approach, as conclusions are drawn from the analysis of the migration patterns of two ssDNA molecules and not from RNA. Using previously characterized mutants of the HCV 5' untranslated regions, we show that contrary to what has been predicted, SSCP migration patterns of DNA amplicons with differences in their nucleotide sequences generated from the full 5' UTR of HCV are not necessarily unique.
dc.fuente.origenWOS
dc.identifier.doi10.1128/JVI.00971-09
dc.identifier.eissn1098-5514
dc.identifier.issn0022-538X
dc.identifier.urihttps://doi.org/10.1128/JVI.00971-09
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/95662
dc.identifier.wosidWOS:000269122300073
dc.issue.numero17
dc.language.isoen
dc.pagina.final9021
dc.pagina.inicio9018
dc.revistaJournal of virology
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleIs Single-Strand Conformation Polymorphism Analysis of the Full 5′ Untranslated Region an Adequate Approach To Study Hepatitis C Virus Quasispecies Distribution?
dc.typeartículo
dc.volumen83
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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