UPTAKE OF MAGNESIUM BY CHROMAFFIN GRANULES INVITRO - ROLE OF THE PROTON ELECTROCHEMICAL GRADIENT

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dc.contributor.authorFIEDLER, J
dc.contributor.authorDANIELS, AJ
dc.date.accessioned2025-01-23T19:43:48Z
dc.date.available2025-01-23T19:43:48Z
dc.date.issued1984
dc.description.abstractThe [bovine] chromaffin granule membrane in vitro is impermeable to protons as well as to Mg2+. When granules are incubated in the presence of the proton ionophore carbonyl cyanide p-trifluoromethoxy-phenylhydrazone or an inhibitor of the granule membrane Mg2+-dependent ATPase, the metal ion is accumulated inside the granules. This accumulation is dependent upon the granule transmembrane potential. The simultaneous presence of the ATPase inhibitor and the proton ionophore markedly increases metal ion incorporation. Mg2+ incorporation is also promoted by nigericin in the presence of K+ or Na+, indicating that Mg2+ accumulation is also dependent upon the transmembrane pH gradient. Concomitant with the Mg2+ accumulation, there is a significant loss of endogenous catecholamines. Mg2+ accumulation is determined by the electrochemical gradient maintained across the membrane. Once the metal ion has accumulated into the granules it displaces catecholamines from their storage sites.
dc.fuente.origenWOS
dc.identifier.eissn1471-4159
dc.identifier.issn0022-3042
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/99827
dc.identifier.wosidWOS:A1984SM50200014
dc.issue.numero5
dc.language.isoen
dc.pagina.final1297
dc.pagina.inicio1291
dc.revistaJournal of neurochemistry
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleUPTAKE OF MAGNESIUM BY CHROMAFFIN GRANULES INVITRO - ROLE OF THE PROTON ELECTROCHEMICAL GRADIENT
dc.typeartículo
dc.volumen42
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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