Proacrosin/acrosin quantification as an indicator of acrosomal integrity in fresh and frozen dog spermatozoa

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dc.contributor.authorCortes, Constanza J.
dc.contributor.authorCodelia, Veronica A.
dc.contributor.authorManosalva, Iris
dc.contributor.authorde Lange, Johanna
dc.contributor.authorDe los Reyes, Monica
dc.contributor.authorMoreno, Ricardo D.
dc.date.accessioned2024-01-10T12:07:43Z
dc.date.available2024-01-10T12:07:43Z
dc.date.issued2006
dc.description.abstractThe scope of the present study was to evaluate the presence and activation of proacrosin/acrosin as a tool to determine the acrosomal status of fresh and frozen/thawed dog spermatozoa. Monoclonal antibody C5F11, directed against human acrosin, cross-reacted with dog spermatozoa and labeled the acrosome of both fresh and frozen/thawed dog spermatozoa. Frozen/thawed spermatozoa had a lesser proportion of labeled spermatozoa than fresh spermatozoa (P < 0.05). When live spermatozoa were labeled with soybean trypsin inhibitor conjugated with Alexa 488 (SBTI-Alexa 488), the proportion of acrosome-labeled fresh spermatozoa was less than frozen/thawed spermatozoa (P < 0.05). By using Western blots and enzymatic activity, frozen/thawed spermatozoa had a greater proportion of active acrosin than fresh spermatozoa. In addition, beta 1,4-galactosyl-transferase (GaIT), a plasma membrane bound protein, remained attached to frozen/thawed spermatozoa. Proacrosin is activated during freezing/thawing of dog spermatozoa, and that proacrosin/acrosin may be a good indicator of acrosomal integrity of frozen/thawed spermatozoa. (c) 2005 Elsevier B.V. All rights reserved.
dc.fechaingreso.objetodigital2024-03-19
dc.format.extent11 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.anireprosci.2005.08.001
dc.identifier.eissn1873-2232
dc.identifier.issn0378-4320
dc.identifier.pubmedidMEDLINE:16169165
dc.identifier.urihttps://doi.org/10.1016/j.anireprosci.2005.08.001
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/76315
dc.identifier.wosidWOS:000237660400015
dc.information.autorucCiencias Biológicas;Codelia VA;S/I;16756
dc.information.autorucCiencias Biológicas;Cortes CJ;S/I;126243
dc.information.autorucEnfermería;De los Reyes M;S/I;83783
dc.information.autorucCiencias Biológicas;Manosalva I;S/I;137056
dc.information.autorucCiencias Biológicas;Moreno RD;S/I;76818
dc.issue.numero1-2
dc.language.isoen
dc.nota.accesoContenido parcial
dc.pagina.final175
dc.pagina.inicio165
dc.publisherELSEVIER
dc.revistaANIMAL REPRODUCTION SCIENCE
dc.rightsacceso restringido
dc.subjectspermatozoa
dc.subjectacrosome
dc.subjectfreezing
dc.subjectfertilization
dc.subjectacrosome reaction
dc.subjectsyntaxin
dc.subjectPOLYSULFATE-BINDING DOMAIN
dc.subjectCAPACITATION-LIKE CHANGES
dc.subjectMOUSE SPERMATOZOA
dc.subjectPOSTTHAW SURVIVAL
dc.subjectACROSIN ACTIVITY
dc.subjectSPERM
dc.subjectACTIVATION
dc.subjectCRYOPRESERVATION
dc.subjectFERTILIZATION
dc.subjectENZYME
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleProacrosin/acrosin quantification as an indicator of acrosomal integrity in fresh and frozen dog spermatozoa
dc.typeartículo
dc.volumen93
sipa.codpersvinculados16756
sipa.codpersvinculados126243
sipa.codpersvinculados83783
sipa.codpersvinculados137056
sipa.codpersvinculados76818
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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