Expression of α<sub>2</sub>-macroglobulin receptor/low density lipoprotein receptor-related protein (LRP) in rat microglial cells

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dc.contributor.authorMarzolo, MP
dc.contributor.authorvon Bernhardi, R
dc.contributor.authorBu, GJ
dc.contributor.authorInestrosa, NC
dc.date.accessioned2025-01-21T01:31:24Z
dc.date.available2025-01-21T01:31:24Z
dc.date.issued2000
dc.description.abstractLow density lipoprotein receptor-related protein (LRP) participates in the uptake and degradation of several ligands implicated in neuronal pathophysiology including apolipoprotein E (apoE), activated alpha(2)-macroglobulin (alpha(2)M*) and beta-amyloid precursor protein (APP). The receptor is expressed in a variety of tissues. in the brain LRP is present in pyramidal-type neurons in cortical and hippocampal regions and in astrocytes that are activated as a result of injury or neoplasmic transformation. As LRP is expressed in the monocyte/macrophage cell system, we were interested in examining whether LRP is expressed in microglia. We isolated glial cells from the brain of neonatal rats and LRP was immunodetected both in microglial cells and in astrocytes expressing glial fibrillar acidic protein (GFAP). Microglial cells were able to bind and internalize LRP-specific ligand, alpha(2)M*. The internalization was inhibitable by RAP, with a Kd of 1.7 nM. The expression of LRP was upregulated by dexamethasone, and down-regulated by lipopolysaccharide (LPS), gamma interferon (IFN-gamma) or a combination of both. LRP was less sensitive to dexamethasone in activated astrocytes than in microglia. We provided the first analysis of LRP expression and regulation in microglia. Our results open the possibility that microglial cells could be related to the participation of LRP and its ligands in different pathophysiological states in brain. (C) 2000 Wiley-Liss, Inc.
dc.description.funderNINDS NIH HHS
dc.fuente.origenWOS
dc.identifier.eissn1097-4547
dc.identifier.issn0360-4012
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/97058
dc.identifier.wosidWOS:000086722100015
dc.issue.numero3
dc.language.isoen
dc.pagina.final411
dc.pagina.inicio401
dc.revistaJournal of neuroscience research
dc.rightsacceso restringido
dc.subjectLRP
dc.subjectmicroglia
dc.subjectalpha-2 macroglobulin
dc.subjectastrocyte
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleExpression of α<sub>2</sub>-macroglobulin receptor/low density lipoprotein receptor-related protein (LRP) in rat microglial cells
dc.typeartículo
dc.volumen60
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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