Extracellular matrix is required for skeletal muscle differentiation rut not myogenin expression

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dc.contributor.authorMelo, F
dc.contributor.authorCarey, DJ
dc.contributor.authorBrandan, E
dc.date.accessioned2025-01-21T01:33:55Z
dc.date.available2025-01-21T01:33:55Z
dc.date.issued1996
dc.description.abstractSkeletal muscle cells are a useful model for studying cell differentiation. Muscle cell differentiation is marked by myoblast proliferation followed by progressive fusion to form large multinucleated myotubes that synthesize muscle-specific proteins and contract spontaneously. The molecular analysis of myogenesis has advanced with the identification of several myogenic regulatory factors, including myod1, myd, and myogenin. These factors regulate each other's expression and that of muscle-specific proteins such as the acetylcholine receptor and acetylcholinesterase (AChE). In order to investigate the role of extracellular matrix (ECM) in myogenesis we have cultured myoblasts (C2C12) in the presence or absence of an exogenous ECM (Matrigel). In addition, we have induced differentiation of myoblasts in the presence or absence of Matrigel and/or chlorate, a specific inhibitor of proteoglycan sulfation. Our results indicated that the formation of fused myotubes and expression of AChE was stimulated by Matrigel. Treatment of myoblasts induced to differentiate with chlorate resulted in an inhibition of cell fusion and AChE activity. Chlorate treatment was also found to inhibit the deposition and assembly of ECM components such fibronectin and laminin. The expression of myogenin mRNA was observed when myoblasts were induced to differentiate, but was unaffected by the presence of Matrigel or by culture of the cells in the presence of chlorate. These results suggest that the expression of myogenin is independent of the presence of ECM, but that the presence of ECM is essential for the formation of myotubes and the expression of later muscle-specific gene products. (C) 1996 Wiley-Liss, Inc.
dc.fuente.origenWOS
dc.identifier.eissn1097-4644
dc.identifier.issn0730-2312
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/97489
dc.identifier.wosidWOS:A1996VA21100011
dc.issue.numero2
dc.language.isoen
dc.pagina.final239
dc.pagina.inicio227
dc.revistaJournal of cellular biochemistry
dc.rightsacceso restringido
dc.subjectskeletal muscle differentiation
dc.subjectchlorate
dc.subjectmyogenin
dc.subjectmatrigel
dc.subjectproteoglycans
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleExtracellular matrix is required for skeletal muscle differentiation rut not myogenin expression
dc.typeartículo
dc.volumen62
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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