CHOLESTEROL CRYSTALLIZATION-PROMOTING ACTIVITY OF AMINOPEPTIDASE-N ISOLATED FROM THE VESICULAR CARRIER OF BILIARY LIPIDS

Simple item page
dc.contributor.authorNUNEZ, L
dc.contributor.authorAMIGO, L
dc.contributor.authorRIGOTTI, A
dc.contributor.authorPUGLIELLI, L
dc.contributor.authorMINGRONE, G
dc.contributor.authorGRECO, AV
dc.contributor.authorNERVI, F
dc.date.accessioned2024-01-10T12:04:37Z
dc.date.available2024-01-10T12:04:37Z
dc.date.issued1993
dc.description.abstractDifferent hydrophobic glycoproteins are associated to native biliary vesicles, which are the major carrier of biliary cholesterol. Some of these proteins promote cholesterol crystallization, a key step in cholesterol gallstone formation. This study was specifically conducted to identify the 130 kDa biliary vesicle-associated glycoprotein and to determine its in vitro effect on the cholesterol crystal formation time. The 130 kDa vesicular glycoprotein was identified as aminopeptidase-N by amino acid sequencing and specific enzymatic assay. Polyclonal antibodies raised against aminopeptidase-N allowed us to determine its concentration in human hepatic bile, which varied from 17.3 to 57.6 mug/ml. Aminopeptidase-N showed a concentration-dependent cholesterol crystallization activity when it was added to supersaturated model bile at a concentration range usually found in native bile. Because of this promoting effect on in vitro cholesterol crystal formation, we suggest that biliary aminopeptidase-N may play a critical role in the pathogenesis of cholesterol gallstone disease.
dc.fechaingreso.objetodigital19-04-2024
dc.format.extent5 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1016/0014-5793(93)80199-5
dc.identifier.issn1873-3468
dc.identifier.pubmedidMEDLINE:8102610
dc.identifier.urihttps://doi.org/10.1016/0014-5793(93)80199-5
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/75845
dc.identifier.wosidWOS:A1993LT84100021
dc.information.autorucActividades Universitarias;Amigo L;S/I;62028
dc.information.autorucMedicina;Nervi F;S/I;99156
dc.information.autorucMedicina;Núñez L;S/I;102374
dc.information.autorucActividades Universitarias;Rigotti A;S/I;68489
dc.issue.numero1-2
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final88
dc.pagina.inicio84
dc.publisherWILEY
dc.revistaFEBS LETTERS
dc.rightsacceso restringido
dc.subjectBILIARY VESICLE
dc.subjectAMINOPEPTIDASE-N
dc.subjectCHOLESTEROL CRYSTALLIZATION
dc.subjectCHOLESTEROL GALLSTONE FORMATION
dc.subjectHUMAN GALLBLADDER BILE
dc.subjectHEPATOCYTE PLASMA-MEMBRANE
dc.subjectDOMAIN-SPECIFIC MARKER
dc.subjectGALLSTONE DISEASE
dc.subjectNUCLEATION TIME
dc.subjectSOLUBILIZATION
dc.subjectPROTEINS
dc.subjectMICELLAR
dc.subjectPHOSPHATIDYLCHOLINE
dc.subjectPATHOGENESIS
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleCHOLESTEROL CRYSTALLIZATION-PROMOTING ACTIVITY OF AMINOPEPTIDASE-N ISOLATED FROM THE VESICULAR CARRIER OF BILIARY LIPIDS
dc.typeartículo
dc.volumen329
sipa.codpersvinculados62028
sipa.codpersvinculados99156
sipa.codpersvinculados102374
sipa.codpersvinculados68489
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
2024-04-19. CHOLESTEROL CRYSTALLIZATION-PROMOTING ACTIVITY OF AMINOPEPTIDASE-N ISOLATED FROM THE VESICULAR CARRIER OF BILIARY LIPIDS.pdf
Size:
2.98 KB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Artículos de revistas