NOREPINEPHRINE INDUCES CA2+ RELEASE FROM INTRACELLULAR STORES IN RAT PINEALOCYTES

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dc.contributor.authorSAEZ, JC
dc.contributor.authorMORENO, AP
dc.contributor.authorSPRAY, DC
dc.date.accessioned2025-01-21T01:35:39Z
dc.date.available2025-01-21T01:35:39Z
dc.date.issued1994
dc.description.abstractIn rat pinealocytes, an increase in intracellular Ca2+ concentration ([Ca2+](i)) due to Ca2+ influx in response to norepinephrine (NE) is a well recognized event involved in regulating several metabolic functions. Since NE also stimulates the metabolism of phosphatidyl inositols in rat pineal gland, it is conceivable that Ca2+ release from intracellular stores also contributes to the NE-induced increase in [Ca2+](i). In this communication, we report that in rat pinealocytes loaded with fura-2, a Ca2+ indicator, NE induced a transient increase in [Ca2+](i) that preceded the known Ca2+ influx. This novel [Ca2+](i) response to NE was detected in pinealocytes bathed with Ca2+-free saline and prevented by TMB-8, a blocker of Ca2+ release from intracellular stores, supporting the notion that the transient NE-induced Ca2+ response was due to Ca2+ release from intracellular stores. In addition, after an extended exposure to NE a new addition of this neurotransmitter did not elicit the phasic Ca2+ response, and application of increasing amounts of NE induced a Ca2+ response that was progressively smaller, suggesting desensitization. Thus, NE is proposed to increase [Ca2+](i) in rat pinealocytes by two mechanisms: (1) phasic release from intracellular stores and (2) tonic influx through a mechanism activated by larger applications of NE than required to evoke the phasic release.
dc.description.funderNIDDK NIH HHS
dc.fuente.origenWOS
dc.identifier.issn0742-3098
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/97705
dc.identifier.wosidWOS:A1994NF26800001
dc.issue.numero2
dc.language.isoen
dc.pagina.final64
dc.pagina.inicio57
dc.revistaJournal of pineal research
dc.rightsacceso restringido
dc.subjectINTRACELLULAR CA2+
dc.subjectNEUROTRANSMITTER
dc.subjectALPHA(1)-ADRENOCEPTORS
dc.subjectDESENSITIZATION
dc.subjectFURA-2
dc.subjectTMB-8
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleNOREPINEPHRINE INDUCES CA2+ RELEASE FROM INTRACELLULAR STORES IN RAT PINEALOCYTES
dc.typeartículo
dc.volumen16
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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