ADAPTIVE REGULATION OF HEPATIC BILE-SALT TRANSPORT - EFFECT OF PROLONGED BILE-SALT DEPLETION IN THE RAT

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dc.contributor.authorACCATINO, L
dc.contributor.authorHONO, J
dc.contributor.authorMALDONADO, M
dc.contributor.authorICARTE, MA
dc.contributor.authorPERSICO, R
dc.date.accessioned2025-01-23T19:24:29Z
dc.date.available2025-01-23T19:24:29Z
dc.date.issued1988
dc.description.abstractExposure of the liver to increased bile salt flux can increase the bile salt maximum secretory rate (SRm), presumably through the induction of new transport sites. The converse, i.e., the down-regulation of SRm upon bile salt deprivation, has not been demonstrated. We examined the effects of bile salt depletion for 24 h and 48 h on taurocholate SRm and bromsulphalein (BSP) SRm, and on [14C]taurocholate binding to isolated liver surface membranes in unrestrained external biliary fistula rats. Taurocholate SRm was significantly decreased by 35% and 51% in 24-h-depleted and 48-h-depleted rats, respectively, compared with control, sham-operated rats. Maximal taurocholate concentration in bile was also significantly lower in the bile-salt-deprived rats. In contrast, BSP SRm was not significantly different between depleted animals and controls. Bile salt depletion for 24 h and 48 h did not significantly alter liver surface membrane protein recovery and membrance enzyme specific activity including Na+ + K+-ATPase. Specific[14C] taurocholate binding to liver surface membranes was significantly decreased by 25% in 24-h-depleted rats compared with control rats. In contrast to taurocholate SRm, bile salt depletion for 48 h did not result in further reduction of specific taurocholate binding sites. This study demonstrates that taurocholate SRm progressively decreased in 24-h- and 48-h-bile salt-depleted rats, this being consistent with adaptive down-regulation of hepatic bile salt transport. This effect is selective, since BSP SRm was unaltered. The depressed taurocholate SRm can be explained at least in part by decreased bile salt receptor density in liver surface membranes. It appears to be unrelated to either a reduction in membrane surface area (membrane protein recovery and enzyme activity were unchanged in bile salt-depleted rats) or altered Na+ electrochemical gradients (Na+ + K+-ATPase activity was not significantly different between bile salt-depleted and control rats).
dc.fuente.origenWOS
dc.identifier.eissn1600-0641
dc.identifier.issn0168-8278
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/99364
dc.identifier.wosidWOS:A1988Q509600011
dc.issue.numero2
dc.language.isoen
dc.pagina.final223
dc.pagina.inicio215
dc.revistaJournal of hepatology
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleADAPTIVE REGULATION OF HEPATIC BILE-SALT TRANSPORT - EFFECT OF PROLONGED BILE-SALT DEPLETION IN THE RAT
dc.typeartículo
dc.volumen7
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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