TRANSCRIPTION INITIATION SITES FOR THE POTATO MITOCHONDRIAL GENE CODING FOR SUBUNIT-9 OF ATP SYNTHASE (ATP9)

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dc.contributor.authorLIZAMA, L
dc.contributor.authorHOLUIGUE, L
dc.contributor.authorJORDANA, X
dc.date.accessioned2025-01-21T01:35:21Z
dc.date.available2025-01-21T01:35:21Z
dc.date.issued1994
dc.description.abstractThe potato mitochondrial atp9 gene has a simple expression pattern. To determine where transcription initiates, primary mitochondrial RNAs were labeled by in vitro capping and hybridized to the 5' flanking sequences of the atp9 gene. A single transcription initiation region was identified. Primer extension and nuclease S1 protection analyses were used to precisely map the transcript 5' termini in this region. These results indicate that transcription initiates at 121-128 bp upstream of the atp9 open reading frame, in a sequence which does not present any homology with proposed consensus sequences for plant mitochondrial promoters. Nuclease S1 protection were also used to map 3' termini 67-71 nucleotides downstream of a putative single-stem loop structure.
dc.fuente.origenWOS
dc.identifier.issn0014-5793
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/97670
dc.identifier.wosidWOS:A1994PA71500016
dc.issue.numero2
dc.language.isoen
dc.pagina.final248
dc.pagina.inicio243
dc.revistaFebs letters
dc.rightsacceso restringido
dc.subjectTRANSCRIPTION INITIATION
dc.subjectATP SYNTHASE SUBUNIT 9
dc.subjectPLANT MITOCHONDRION
dc.subjectGUANYLYL TRANSFERASE
dc.subjectSOLANUM TUBEROSUM
dc.subject.ods13 Climate Action
dc.subject.odspa13 Acción por el clima
dc.titleTRANSCRIPTION INITIATION SITES FOR THE POTATO MITOCHONDRIAL GENE CODING FOR SUBUNIT-9 OF ATP SYNTHASE (ATP9)
dc.typeartículo
dc.volumen349
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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