YEAST RNA-POLYMERASE .1. A EUKARYOTIC ZINC METALLOENZYME

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dc.contributor.authorAULD, DS
dc.contributor.authorATSUYA, I
dc.contributor.authorCAMPINO, C
dc.contributor.authorVALENZUELA, P
dc.date.accessioned2025-01-23T19:45:00Z
dc.date.available2025-01-23T19:45:00Z
dc.date.issued1976
dc.description.abstractMicrowave excitation spectrometry and metal binding inhibition studies showed that Zn is a catalytically essential component of the highly purified RNA polymerase I from yeast, the 1st eukaryotic RNA polymerase I available in quantities sufficient for such studies. It contained 2.4 g-atom of Zn based on a MW of 6.5 .times. 105. Cu, Fe, Mn and Mg were absent, i.e., below the limits of detection, 10-3-10-14 g-atoms. A number of derivatives of 1,10-phenanthroline reversibly inhibited the polymerase catalyzed reaction, apparently by forming a ternary polymerase.cntdot.Zn.cntdot.OP complex while the nonchelating isomer, 1,7-phenanthroline, was ineffective.
dc.fuente.origenWOS
dc.identifier.eissn1090-2104
dc.identifier.issn0006-291X
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/100179
dc.identifier.wosidWOS:A1976BN09600038
dc.issue.numero2
dc.language.isoen
dc.pagina.final554
dc.pagina.inicio548
dc.revistaBiochemical and biophysical research communications
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleYEAST RNA-POLYMERASE .1. A EUKARYOTIC ZINC METALLOENZYME
dc.typeartículo
dc.volumen69
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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