Lipid-Encapsuled Grape Tannins Prevent Oxidative-Stress-Induced Neuronal Cell Death, Intracellular ROS Accumulation and Inflammation

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dc.article.number1928
dc.contributor.authorDiaz H.S.
dc.contributor.authorRios-Gallardo A.
dc.contributor.authorOrtolani D.
dc.contributor.authorDiaz-Jara E.
dc.contributor.authorFlores M.J.
dc.contributor.authorVera I.
dc.contributor.authorRio R.D.
dc.contributor.authorMonasterio A.
dc.contributor.authorOsorio F.
dc.contributor.authorOrtiz F.C.
dc.contributor.authorBrossard N.
dc.date.accessioned2024-01-10T12:37:16Z
dc.date.available2024-01-10T12:37:16Z
dc.date.issued2022
dc.description.abstract© 2022 by the authors.The central nervous system (CNS) is particularly vulnerable to oxidative stress and inflammation, which affect neuronal function and survival. Nowadays, there is great interest in the development of antioxidant and anti-inflammatory compounds extracted from natural products, as potential strategies to reduce the oxidative/inflammatory environment within the CNS and then preserve neuronal integrity and brain function. However, an important limitation of natural antioxidant formulations (mainly polyphenols) is their reduced in vivo bioavailability. The biological compatible delivery system containing polyphenols may serve as a novel compound for these antioxidant formulations. Accordingly, in the present study, we used liposomes as carriers for grape tannins, and we tested their ability to prevent neuronal oxidative stress and inflammation. Cultured catecholaminergic neurons (CAD) were used to establish the potential of lipid-encapsulated grape tannins (TLS) to prevent neuronal oxidative stress and inflammation following an oxidative insult. TLS rescued cell survival after H2O2 treatment (59.4 ± 8.8% vs. 90.4 ± 5.6% H2O2 vs. TLS+ H2O2; p < 0.05) and reduced intracellular ROS levels by ~38% (p < 0.05), despite displaying negligible antioxidant activity in solution. Additionally, TLS treatment dramatically reduced proinflammatory cytokines’ mRNA expression after H2O2 treatment (TNF-α: 400.3 ± 1.7 vs. 7.9 ± 1.9-fold; IL-1β: 423.4 ± 1.3 vs. 12.7 ± 2.6-fold; p < 0.05; H2O2 vs. TLS+ H2O2, respectively), without affecting pro/antioxidant biomarker expression, suggesting that liposomes efficiently delivered tannins inside neurons and promoted cell survival. In conclusion, we propose that lipid-encapsulated grape tannins could be an efficient tool to promote antioxidant/inflammatory cell defense.
dc.description.funderBASAL
dc.description.funderBasal Center of Excellence in Aging and Regeneration
dc.description.funderCenter of Applied Ecology and Sustainability CONICYT
dc.description.funderChilean Agency of Research and Development
dc.description.funderFONDECYT
dc.description.funderANID
dc.description.funderPIA
dc.fechaingreso.objetodigital2024-05-23
dc.fuente.origenScopus
dc.identifier.doi10.3390/antiox11101928
dc.identifier.eissn2076-63921
dc.identifier.issn20763921
dc.identifier.scopusidSCOPUS_ID:85140469673
dc.identifier.urihttps://doi.org/10.3390/antiox11101928
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/76802
dc.identifier.wosidWOS:000872038400001
dc.information.autorucFacultad de Agronomía e Ingeniería Forestal; Brossard Aravena, Natalia Daniela; S/I; 149592
dc.issue.numero10
dc.language.isoen
dc.nota.accesoContenido completo
dc.publisherMDPI
dc.relation.ispartofAntioxidants
dc.revistaAntioxidants
dc.rightsacceso abierto
dc.subjectliposomes
dc.subjectnatural products
dc.subjectneuroprotection
dc.subjectoxidative stress
dc.subjectpolyphenols
dc.subject.ods03 Good health and well-being
dc.subject.odspa03 Salud y bienestar
dc.titleLipid-Encapsuled Grape Tannins Prevent Oxidative-Stress-Induced Neuronal Cell Death, Intracellular ROS Accumulation and Inflammation
dc.typeartículo
dc.volumen11
sipa.codpersvinculados149592
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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