Purification and biochemical characterization of a trypsin-like enzyme present in the sperm of the rock shrimp, <i>Rhynchocinetes typus</i>

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dc.contributor.authorBustamante, E
dc.contributor.authorPalomino, J
dc.contributor.authorAmoroso, A
dc.contributor.authorMoreno, R
dc.contributor.authorBarros, C
dc.date.accessioned2025-01-21T01:30:43Z
dc.date.available2025-01-21T01:30:43Z
dc.date.issued2001
dc.description.abstractThe aim of the present work was to isolate, purify and characterize a trypsin-like enzyme from the sperm of the rock shrimp, Rhynchocinetes typus. Sperm proteins were extracted with 1 mM HCl in 10% glycerol at pH 3.0. Purification of the trypsin-like substance was effected by affinity chromatography using SBTI-agarose, yielding a specific activity on BAEE as substrate of 787 U/mg, with a recovery rate of 34%. Enzymatic activity was maximal at 27 degreesC, pH of 8.0, 50 mM Ca2+ and 30 mM Mg2+. One hundred percent inhibition of enzymatic activity was obtained at 0.05 mM Zn2+. Kinetic analysis showed that the K-M on BAEE as substrate at pH 8.0 was 2.5 x 10(-5) M and the V-MAX reached was 198 U. It was also found that the enzyme had a substrate inhibition at concentrations higher than 0.06 mM of BAEE. These findings suggest that thin enzyme has similar characteristics to other trypsin-like enzymes including acrosin.
dc.fuente.origenWOS
dc.identifier.issn0168-8170
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/96869
dc.identifier.wosidWOS:000171879700002
dc.issue.numero3
dc.language.isoen
dc.pagina.final181
dc.pagina.inicio175
dc.revistaInvertebrate reproduction & development
dc.rightsacceso restringido
dc.subjectsperm
dc.subjecttrypsin-like
dc.subjectenzyme
dc.subjectshrimp
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titlePurification and biochemical characterization of a trypsin-like enzyme present in the sperm of the rock shrimp, <i>Rhynchocinetes typus</i>
dc.typeartículo
dc.volumen39
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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