SCANNING ELECTRON-MICROSCOPE STUDY OF INVITRO PREPENETRATION GAMETE INTERACTIONS

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dc.contributor.authorJEDLICKI, A
dc.contributor.authorBARROS, C
dc.date.accessioned2025-01-23T19:27:18Z
dc.date.available2025-01-23T19:27:18Z
dc.date.issued1985
dc.description.abstractHamster and mouse capacitated spermatozoa were interacted in vitro with hamster and mouse eggs in homologous and heterologous combinations. Also, fertilized and trypsin treated unfertilized hamster eggs and unfertilized rat eggs were made to interact with capacitated hamster spermatozoa. The surface of the zona pellucida was then examined with the scanning electron microscope. Sperm attachment, followed by sperm binding and penetration through the zona pellucida, was observed only when homologous gamete combinations were used. Binding of the spermatozoa to the zona was homologous gamete combinations were used. Binding of the spermatozoa to the zona was evidenced by the lytic effect of the acrosomal enzymes on the zona substance. When fertilized eggs and trypsin-treated unfertilized hamster eggs were mixed with capacitated hamster spermatozoa as well as in the heterologous gamete combinations, the spermatozoa were able to establish attachment but not binding. Under these conditions the outer surface of the zona pellucida was never found to have penetration tracks made by the spermatoza. Failure of heterologous spermatozoa to cross the foreign zona pellucida is believed to be associated with the inability of the foreign spermatozoa to establish binding and to the inability of their acrosomal enzymes to digest the zona. A similar mechanism is believed to work in zona-reacted and in trypsin-treated hamster eggs inseminated in vitro with homologous spermatozoa.
dc.fuente.origenWOS
dc.identifier.issn0148-7280
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/99710
dc.identifier.wosidWOS:A1985AEC5800002
dc.issue.numero2
dc.language.isoen
dc.pagina.final131
dc.pagina.inicio121
dc.revistaGamete research
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleSCANNING ELECTRON-MICROSCOPE STUDY OF INVITRO PREPENETRATION GAMETE INTERACTIONS
dc.typeartículo
dc.volumen11
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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