Activity of the human immunodeficiency virus type 1 cell cycle-dependent internal ribosomal entry site is modulated by IRES trans-acting factors

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dc.contributor.authorVallejos, Maricarmen
dc.contributor.authorDeforges, Jules
dc.contributor.authorPlank, Terra Dawn M.
dc.contributor.authorLetelier, Alejandro
dc.contributor.authorRamdohr, Pablo
dc.contributor.authorAbraham, Christopher G.
dc.contributor.authorValiente Echeverria, Fernando
dc.contributor.authorKieft, Jeffrey S.
dc.contributor.authorSargueil, Bruno
dc.contributor.authorLopez Lastra, Marcelo
dc.date.accessioned2024-01-10T13:10:52Z
dc.date.available2024-01-10T13:10:52Z
dc.date.issued2011
dc.description.abstractThe 5' leader of the human immunodeficiency virus type 1 (HIV-1) genomic RNA harbors an internal ribosome entry site (IRES) that is functional during the G2/M phase of the cell cycle. Here we show that translation initiation mediated by the HIV-1 IRES requires the participation of trans-acting cellular factors other than the canonical translational machinery. We used 'standard' chemical and enzymatic probes and an 'RNA SHAPE' analysis to model the structure of the HIV-1 5' leader and we show, by means of a footprinting assay, that G2/M extracts provide protections to regions previously identified as crucial for HIV-1 IRES activity. We also assessed the impact of mutations on IRES function. Strikingly, mutations did not significantly affect IRES activity suggesting that the requirement for pre-formed stable secondary or tertiary structure within the HIV-1 IRES may not be as strict as has been described for other viral IRESes. Finally, we used a proteomic approach to identify cellular proteins within the G2/M extracts that interact with the HIV-1 5' leader. Together, data show that HIV-1 IRES-mediated translation initiation is modulated by cellular proteins.
dc.description.funderFONDECYT
dc.description.funderProyecto Nucleo Milenio
dc.description.funderCNRS-CONICYT
dc.description.funderEscuela de Medicina de la Pontificia Universidad Catolica de Chile
dc.description.funderCONICYT
dc.description.funderAgence Nationale de Recherche contre le SIDA (ANRS) for B.S. Laboratory work
dc.description.funder'ATIP' from CNRS
dc.description.funderSIDACTION
dc.description.funderNational Institutes of Health
dc.description.funderle Ministere de l'Education et de la Recherche
dc.description.funderVRI-Pontificia Universidad Catolica de Chile
dc.description.funderComision Nacional de Investigacion Cientifica y Tecnologica, Gobierno de Chile, CONICYT through grant FONDECYT
dc.description.funderCNRS-DRI
dc.description.funderNATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
dc.fechaingreso.objetodigital2024-04-26
dc.format.extent15 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1093/nar/gkr189
dc.identifier.eissn1362-4962
dc.identifier.issn0305-1048
dc.identifier.pubmedidMEDLINE:21482538
dc.identifier.urihttps://doi.org/10.1093/nar/gkr189
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/77951
dc.identifier.wosidWOS:000293919600037
dc.information.autorucCiencias Biológicas;Letelier A ;S/I;12728
dc.information.autorucMedicina;Lopez-Lastra M;S/I;84823
dc.information.autorucCiencias Biológicas;Ramdohr P ;S/I;119722
dc.information.autorucMedicina;Valiente F;S/I;1006402
dc.information.autorucCiencias Biológicas;Vallejos M ;S/I;132558
dc.issue.numero14
dc.language.isoen
dc.nota.accesocontenido completo
dc.pagina.final6200
dc.pagina.inicio6186
dc.publisherOXFORD UNIV PRESS
dc.revistaNUCLEIC ACIDS RESEARCH
dc.rightsacceso abierto
dc.subjectHIV-1 TAT PROTEIN
dc.subjectRNA SECONDARY STRUCTURE
dc.subjectMESSENGER-RNA
dc.subjectIN-VITRO
dc.subjectTRANSLATION INITIATION
dc.subjectTOPOISOMERASE-I
dc.subjectPOLIOVIRUS RNA
dc.subjectREGION
dc.subjectCAP
dc.subjectCLEAVAGE
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleActivity of the human immunodeficiency virus type 1 cell cycle-dependent internal ribosomal entry site is modulated by IRES trans-acting factors
dc.typeartículo
dc.volumen39
sipa.codpersvinculados12728
sipa.codpersvinculados84823
sipa.codpersvinculados119722
sipa.codpersvinculados1006402
sipa.codpersvinculados132558
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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