Photosynthetic Solutions for Organ Perfusion Based on Microalgae and Cyanobacteria Display Differential In Vitro and In Vivo Features for Intravascular Oxygenation

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dc.catalogadorpva
dc.contributor.authorBecerra, Daniela
dc.contributor.authorVargas Torres, Valentina Isabel
dc.contributor.authorVeloso Giménez, Valentina del Carmen
dc.contributor.authorGallardo Agüero, Daniela
dc.contributor.authorMiranda Cárdenas, Miguel Angel
dc.contributor.authorHernández Pavez, Valentina
dc.contributor.authorGonzalez Quezada, Nicolás Marcelo Orlando
dc.contributor.authorSan Martín, Sebastián
dc.contributor.authorBoric P., Mauricio
dc.contributor.authorEgaña, José T.
dc.date.accessioned2025-08-29T15:43:23Z
dc.date.available2025-08-29T15:43:23Z
dc.date.issued2025
dc.description.abstractThe delivery of photosynthetic microorganisms has emerged as a strategy for tissue oxygenation, offering a promising approach to treat several hypoxic conditions. Among these, intravascular photosynthesis has been proposed for ex vivo organ preservation; however, the most suitable photosynthetic microorganisms and their behavior during intravascular perfusion remain to be fully elucidated. Therefore, this study evaluates key properties of photosynthetic solutions for organ perfusion, based on the microalgaeChlamydomonas reinhardtii and the cyanobacterium Synechococcus elongatus. In vitro characterization showed that both microorganisms maintained viability, morphology, and oxygen production capacity in a Ringer’s lactate-based medium for at least 24 h, with both photosynthetic solutions exhibiting rheological properties compatible with organ perfusion. In vivo perfusion of rat kidneys demonstrates sustained hemodynamic stability, with S. elongatus showing lower variability in vascular resistance. Histological analysis revealed significant retention of both microorganisms within renal structures, with S. elongatus inducing less tubular damage. Additionally, biocompatibility assays with human endothelial cells and zebrafish larvae showed no significant cytotoxic effects of the photosynthetic solutions. These findings support the feasibility of using photosynthetic microorganisms for intravascular photosynthesis, highlighting S. elongatus as particularly promising due to its lower oxygen consumption in darkness and reduced tissue damage after perfusion. This work provides significant insights toward the development of biologically active perfusion systems for innovative preservation strategies for organ transplantation.
dc.fechaingreso.objetodigital2025-08-29
dc.format.extent16 páginas
dc.fuente.origenORCID
dc.identifier.doi10.1021/acsabm.5c01137
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/105369
dc.identifier.urihttps://doi.org/10.1021/acsabm.5c01137
dc.information.autorucInstituto de Ingeniería Biológica y Médica; Vargas Torres, Valentina Isabel; S/I; 247359
dc.information.autorucEscuela de Ingeniería; Veloso Giménez, Valentina del Carmen; S/I; 1049917
dc.information.autorucEscuela de Ingeniería; Miranda Cárdenas, Miguel Angel; S/I; 1092360
dc.information.autorucEscuela de Química; Gonzalez Quezada, Nicolás Marcelo Orlando; S/I; 1066474
dc.information.autorucFacultad de Ciencias Biológicas; Boric P., Mauricio; 0000-0001-6735-6675; 56355
dc.information.autorucInstituto de Ingeniería Biológica y Médica; Egaña, José T.; 0000-0003-2624-6992; 1018715
dc.issue.numero8
dc.language.isoen
dc.nota.accesocontenido completo
dc.pagina.final7448
dc.pagina.inicio7433
dc.publisherAmerican Chemical Society
dc.revistaACS Applied Bio Materials
dc.rightsacceso abierto
dc.rights.licenseAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectPhotosynthetic therapies
dc.subjectOrgan perfusion
dc.subjectPhotosynthetic microorganisms
dc.subjectChlamydomonas reinhardtii
dc.subjectSynechococcus elongatus
dc.subjectBiological oxygen carriers
dc.subjectRenal preservation
dc.subject.ddc610
dc.subject.deweyMedicina y saludes_ES
dc.subject.ods03 Good health and well-being
dc.subject.odspa03 Salud y bienestar
dc.titlePhotosynthetic Solutions for Organ Perfusion Based on Microalgae and Cyanobacteria Display Differential In Vitro and In Vivo Features for Intravascular Oxygenation
dc.typeartículo
dc.volumen8
sipa.codpersvinculados247359
sipa.codpersvinculados1049917
sipa.codpersvinculados1092360
sipa.codpersvinculados1066474
sipa.codpersvinculados56355
sipa.codpersvinculados1018715
sipa.trazabilidadORCID;2025-08-22
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