Characterization of a Novel <i>Drosophila</i> SERT Mutant: Insights on the Contribution of the Serotonin Neural System to Behaviors

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dc.contributor.authorHidalgo, Sergio
dc.contributor.authorMolina-Mateo, Daniela
dc.contributor.authorEscobedo, Pia
dc.contributor.authorZarate, Rafaella V.
dc.contributor.authorFritz, Elsa
dc.contributor.authorFierro, Angelica
dc.contributor.authorPerez, Edwin G.
dc.contributor.authorIturriaga-Vasquez, Patricio
dc.contributor.authorReyes-Parada, Miguel
dc.contributor.authorVaras, Rodrigo
dc.contributor.authorFuenzalida-Uribe, Nicolas
dc.contributor.authorCampusano, Jorge M.
dc.date.accessioned2025-01-23T21:24:44Z
dc.date.available2025-01-23T21:24:44Z
dc.date.issued2017
dc.description.abstractA better comprehension on how different molecular components of the serotonergic system contribute to the adequate regulation of behaviors in animals is essential in the interpretation on how they are involved in neuropsychiatric and pathological disorders. It is possible to study these components in "simpler" animal models including the fly Drosophila melanogaster, given that most of the components of the serotonergic system are conserved between vertebrates and invertebrates. Here we decided to advance our understanding on how the serotonin plasma membrane transporter (SERT) contributes to serotonergic neurotransmission and behaviors in Drosophila. In doing this, we characterized for the first time a mutant for Drosophila SERT (dSERT) and additionally used a highly selective serotonin-releasing drug, 4-methylthioamphetamine (4-MTA), whose mechanism of action involves the SERT protein. Our results show that dSERT mutant animals exhibit an increased survival rate in stress conditions, increased basal motor behavior, and decreased levels in an anxiety-related parameter, centrophobism. We also show that 4-MTA increases the negative chemotaxis toward a strong aversive odorant, benzaldehyde. Our neurochemical data suggest that this effect is mediated by dSERT and depends on the 4-MTA-increased release of serotonin in the fly brain. Our in silico data support the idea that these effects are explained by specific interactions between 4-MTA and dSERT. In sum, our neurochemical, in silico, and behavioral analyses demonstrate the critical importance of the serotonergic system and particularly dSERT functioning in modulating several behaviors in Drosophila.
dc.fuente.origenWOS
dc.identifier.doi10.1021/acschemneuro.7b00089
dc.identifier.issn1948-7193
dc.identifier.urihttps://doi.org/10.1021/acschemneuro.7b00089
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/101315
dc.identifier.wosidWOS:000413502700012
dc.issue.numero10
dc.language.isoen
dc.pagina.final2179
dc.pagina.inicio2168
dc.revistaAcs chemical neuroscience
dc.rightsacceso restringido
dc.subjectSERT
dc.subjectDrosophila
dc.subjectamine release
dc.subjectolfaction
dc.subjectmotor behavior
dc.subjectcentrophobism
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleCharacterization of a Novel <i>Drosophila</i> SERT Mutant: Insights on the Contribution of the Serotonin Neural System to Behaviors
dc.typeartículo
dc.volumen8
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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