First Report of Monilinia fructicola causing brown rot on stored Japanese plum fruit in Chile.

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dc.catalogadoraba
dc.contributor.authorLatorre G., Bernardo
dc.contributor.authorDíaz Ulloa, Gonzalo Alberto
dc.contributor.authorValencia Díaz, Ana Luisa
dc.contributor.authorNaranjo, P.
dc.contributor.authorFerrada, Enrique E.
dc.contributor.authorTorres, R.
dc.contributor.authorZoffoli, Juan Pablo
dc.date.accessioned2025-03-07T19:49:25Z
dc.date.available2025-03-07T19:49:25Z
dc.date.issued2014
dc.description.abstractIn autumn 2013, fruit of Japanese plum (Prunus salicina) cvs. Angelino and Black Kat developed an unusual brown and soft rot after 2 months in cold storage (0°C) on nearly 1% of the fruit. Fruit showed small, circular, light brown spots that eventually destroyed the entire fruit. Small sporodochia appeared on the fruit surface. Fruit was harvested from orchards located near San Francisco de Mostazal (33°59′ S, 70°41′ W), Chile. Small pieces of diseased tissue were selected from margins of lesions of surface disinfected (96% ethanol) fruit (n = 7) and placed on acidified potato dextrose agar (PDA) plates for 5 days at 20°C. Light brown colonies with even margins and concentric rings of spores were obtained. The conidia of five isolates were one-celled, hyaline, lemon-shaped, (min. 10.7) 14.9 ± 1.5 (max. 18.6) × (min. 8.1) 9.4 ± 0.8 (max. 10.8) μm (n = 30), and borne in branched monilioid chains. This fungus was identified as Monilinia fructicola (G. Winter) Honey (1). Identification was confirmed by amplifying and sequencing the ribosomal ITS1-5.8S-ITS2 region using ITS1 and ITS4 primers (3). BLAST analysis of Chilean plum isolates (GenBank Accession Nos. KF148610 and KF148611) were 99 to 100% identical to isolates of M. fructicola originating from the United States (DQ314727 and HQ846966, respectively) and 100% identical to the first Chilean isolate (JN001480) found in nectarines originating from California at the supermarkets in Santiago in June 2009. Koch's postulates were fulfilled by reproducing brown rot symptoms on mature wounded Japanese plums cv. Angelino (n = 8) inoculated with 10 μl of a conidial suspension (105 conidia/ml) or with a mycelium plug (5-mm diameter). After 2 days in humid chambers (>80% relative humidity) at 25°C, all inoculated fruit developed brown rot symptoms with necrotic lesion means of 15.8 and 21.5 mm in diameter in fruit inoculated with conidia and mycelium, respectively. Non-inoculated control fruit remained healthy. Re-isolations were performed on PDA and the presence of M. fructicola was morphologically confirmed in 100% of the symptomatic fruits. To our knowledge, this is the first report demonstrating the presence of M. fructicola causing brown rot in stored Japanese plums in Chile after its first interception in 2009 in Chile, suggesting that this pathogen has been established in the field. Currently, M. fructicola is a quarantine organism under official control, restricted to Prunus orchards between Santiago and Nancagua in central Chile (2).
dc.description.funderUnidad Académica
dc.fuente.origenHistorial Académico
dc.identifier.doi10.1094/PDIS-06-13-0647-PDN
dc.identifier.eissn1943-7692
dc.identifier.issn0191-2917
dc.identifier.scopusid2-s2.0-84889687595
dc.identifier.urihttps://doi.org/10.1094/PDIS-06-13-0647-PDN
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/102447
dc.identifier.wosidWOS:000328656900039
dc.information.autorucFacultad de Agronomía e Ingenieria Forestal; Latorre G., Bernardo; S/I; 98977
dc.information.autorucFacultad de Agronomía e Ingenieria Forestal; Valencia Díaz, Ana Luisa; S/I; 192020
dc.information.autorucFacultad de Agronomía e Ingenieria Forestal; Ferrada, Enrique E.; S/I; 218162
dc.information.autorucFacultad de Agronomía e Ingenieria Forestal; Zoffoli, Juan Pablo; 0000-0003-0455-8477; 58311
dc.information.autorucFacultad de Agronomía e Ingenieria Forestal; Díaz Ulloa, Gonzalo Alberto; S/I; 189542
dc.issue.numero1
dc.language.isoen
dc.nota.accesocontenido parcial
dc.revistaPlant Disease
dc.rightsacceso restringido
dc.subject.ddc600
dc.subject.deweyTecnologíaes_ES
dc.subject.ods03 Good health and well-being
dc.subject.odspa03 Salud y bienestar
dc.titleFirst Report of Monilinia fructicola causing brown rot on stored Japanese plum fruit in Chile.
dc.typeartículo
dc.volumen98
sipa.codpersvinculados121769
sipa.codpersvinculados11594
sipa.codpersvinculados86705
sipa.codpersvinculados98977
sipa.codpersvinculados192020
sipa.codpersvinculados218162
sipa.codpersvinculados58311
sipa.codpersvinculados189542
sipa.trazabilidadHistorial Académico;09-07-2021
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