Characterization of Botrytis cinerea from table grapes in Chile using RAPD-PCR

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dc.contributor.authorThompson, JR
dc.contributor.authorLatorre, BA
dc.date.accessioned2024-01-10T13:46:40Z
dc.date.available2024-01-10T13:46:40Z
dc.date.issued1999
dc.description.abstractRandom amplified polymorphic DNA (RAPD) analysis was performed on 29 isolates of Botrytis cinerea Pers. ex Fr. isolated from table grapes (Vitis vinifera L.) and other crops in Chile with 29 decaprimers. No single primer was found to differentiate either the host or the geographical origin of each of the B. cinerea isolates tested. The DNA profiles obtained, particularly with primers OPA4 and OPA11, distinguished isolates of B. cinerea from other epiphyte fungi found on table grapes, including Alternaria alternata, Aspergillus niger; Cladosporium herbarum, Epiccocum nigrum; Rhizopus stolonifer, a Penicillium sp., and yeasts (Cryptococcus laurentii, Rhodotorula glutinis, and Saccharomyces cerevisiae). Regardless of host origin, primers OPA4 and OPA11 amplified a strong fragment of 1.2 kilobases (kb) and two fragments of 1.10 and 0.7 kb, respectively. These DNA fragments were obtained even when only one conidium of B. cinerea was in the test sample. Three main groups were clearly defined based on the genetic similarities found in additional RAPD analysis with 19 arbitrary decaprimers and 15 selected isolates of B. cinerea. The overall similarity coefficients (SC) between the groups obtained ranged from 0.326 to 0.891. Interestingly, all isolates from table grapes were included in group I(SC: 0.761 to 0.826), isolates from apple and tomato were in group II (SC: 0.739 to 0.848), while isolates from blueberry were either in group I (SC: 0.804) or III (SC: 0.673). Consequently, the genetic variability determined by RAPD analysis among these B. cinerea isolates suggested a possible host:pathogen relationship. However, further research is needed to clarify its pathological significance.
dc.fechaingreso.objetodigital2024-04-29
dc.format.extent5 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1094/PDIS.1999.83.12.1090
dc.identifier.issn0191-2917
dc.identifier.pubmedidMEDLINE:30841128
dc.identifier.urihttps://doi.org/10.1094/PDIS.1999.83.12.1090
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/79187
dc.identifier.wosidWOS:000083850100002
dc.information.autorucAgronomía e Ing. Forestal;Latorre B;S/I;98977
dc.issue.numero12
dc.language.isoen
dc.nota.accesocontenido completo
dc.pagina.final1094
dc.pagina.inicio1090
dc.publisherAMER PHYTOPATHOLOGICAL SOC
dc.revistaPLANT DISEASE
dc.rightsacceso abierto
dc.subjectapple
dc.subjectblueberry
dc.subjectBotryotinia fuckeliana
dc.subjectBotrytis bunch rot
dc.subjectgray mold
dc.subjecttomato
dc.subjectDNA POLYMORPHISMS
dc.subjectMARKERS
dc.subject.ods13 Climate Action
dc.subject.ods02 Zero Hunger
dc.subject.odspa13 Acción por el clima
dc.subject.odspa02 Hambre cero
dc.titleCharacterization of Botrytis cinerea from table grapes in Chile using RAPD-PCR
dc.typeartículo
dc.volumen83
sipa.codpersvinculados98977
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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