<i>bcpmr1</i> encodes a P-type Ca<SUP>2+</SUP>/Mn<SUP>2+</SUP>-ATPase mediating cell-wall integrity and virulence in the phytopathogen <i>Botrytis cinerea</i>

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dc.contributor.authorPlaza, Veronica
dc.contributor.authorLaguees, Yanssuy
dc.contributor.authorCarvajal, Mauro
dc.contributor.authorPerez-Garcia, Luis A.
dc.contributor.authorMora-Montes, Hector M.
dc.contributor.authorCanessa, Paulo
dc.contributor.authorLarrondo, Luis F.
dc.contributor.authorCastillo, Luis
dc.date.accessioned2025-01-23T21:38:25Z
dc.date.available2025-01-23T21:38:25Z
dc.date.issued2015
dc.description.abstractThe cell wall of fungi is generally composed of an inner skeletal layer consisting of various polysaccharides surrounded by a layer of glycoproteins. These usually contain both N- and O-linked oligosaccharides, coupled to the proteins by stepwise addition of mannose residues by mannosyltransferases in the endoplasmic reticulum and the Golgi apparatus. In yeast, an essential luminal cofactor for these mannosyltransferases is Mn2+ provided by the Ca2+/Mn2+-ATPase known as Pmr1. In this study, we have identified and characterized the Botrytis cinerea pmr1 gene, the closest homolog of yeast PMR1. We hypothesized that bcpmr1 also encodes a Ca2+/Mn2+-ATPase that plays an important role in the protein glycosylation pathway. Phenotypic analysis showed that bcpmr1 null mutants displayed a significant reduction in conidial production, radial growth and diameter of sclerotia. Significant alterations in hyphal cell wall composition were observed including a 83% decrease of mannan levels and an increase in the amount of chitin and glucan. These changes were accompanied by a hypersensitivity to cell wall-perturbing agents such as Calcofluor white, Congo red and zymolyase. Importantly, the Delta bcpmr1 mutant showed reduced virulence in tomato (leafs and fruits) and apple (fruits) and reduced biofilm formation. Together, our results highlight the importance of bcpmr1 for protein glycosylation, cell wall structure and virulence of B. cinerea. (C) 2015 Elsevier Inc. All rights reserved.
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.fgb.2015.01.012
dc.identifier.eissn1096-0937
dc.identifier.issn1087-1845
dc.identifier.urihttps://doi.org/10.1016/j.fgb.2015.01.012
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/101592
dc.identifier.wosidWOS:000353527200005
dc.language.isoen
dc.pagina.final46
dc.pagina.inicio36
dc.revistaFungal genetics and biology
dc.rightsacceso restringido
dc.subjectBotrytis cinerea
dc.subjectGlycosylation
dc.subjectCell wall
dc.subjectPMR1
dc.title<i>bcpmr1</i> encodes a P-type Ca<SUP>2+</SUP>/Mn<SUP>2+</SUP>-ATPase mediating cell-wall integrity and virulence in the phytopathogen <i>Botrytis cinerea</i>
dc.typeartículo
dc.volumen76
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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