Gap junctional communication coordinates vasopressin-induced glycogenolysis in rat hepatocytes

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dc.contributor.authorEugenín, EA
dc.contributor.authorGonzález, H
dc.contributor.authorSáez, CG
dc.contributor.authorSáez, JC
dc.date.accessioned2025-01-21T01:32:38Z
dc.date.available2025-01-21T01:32:38Z
dc.date.issued1998
dc.description.abstractBecause hepatocytes communicate via gap junctions, it has been proposed that Ca2+ waves propagate through this pathway and in the process activate Ca2+-dependent cellular responses. We tested this hypothesis by measuring vasopressin-induced glycogenolysis in shortterm cultures of rat hepatocytes. A 15-min vasopressin (10(-8) M) stimulation induced a reduction of glycogen content that reached a maximum 1-3 h later. Gap junction blockers, octanol or 18 alpha-glycyrrhetinic acid, reduced the effect by 70%. The glycogenolytic response induced by Ca2+ ionophore 8-bromo-A-21387, which acts on each hepatocyte, was not affected by gap junction blockers. Moreover, the vasopressin-induced glycogenolysis was lower (70%) in dispersed than in reaggregated hepatocytes and in dispersed hepatocytes was not affected by gap junction blockers. In hepatocytes reaggregated in the presence of a synthetic peptide homologous to a domain of the extracellular loop I of the main hepatocyte gap junctional protein, vasopressin-induced glycogenolysis and incidence of dye coupling were drastically reduced. Moreover, gap junctional communication was detected between reaggregated cells, suggesting that hepatocytes with different vasopressin receptor densities become coupled to each other. The vasopressin-induced effect was not affected by suramin, ruling out ATP as a paracrine mediator. We propose that gap junctions allow for a coordinated vasopressin-induced glycogenolytic response despite the heterogeneity among hepatocytes.
dc.fuente.origenWOS
dc.identifier.issn0193-1857
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/97297
dc.identifier.wosidWOS:000074202600020
dc.issue.numero6
dc.language.isoen
dc.pagina.finalG1116
dc.pagina.inicioG1109
dc.revistaAmerican journal of physiology-gastrointestinal and liver physiology
dc.rightsacceso restringido
dc.subjectcell-to-cell communication
dc.subjectcultured hepatocytes
dc.subjectglycogen content
dc.subjectoctanol
dc.subject18 alpha-glycyrrhetinic acid
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleGap junctional communication coordinates vasopressin-induced glycogenolysis in rat hepatocytes
dc.typeartículo
dc.volumen274
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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