Ecm29-Dependent Proteasome Localization Regulates Cytoskeleton Remodeling at the Immune Synapse

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dc.contributor.authorIbanez-Vega, Jorge
dc.contributor.authorDel Valle, Felipe
dc.contributor.authorSaez, Juan Jose
dc.contributor.authorGuzman, Fanny
dc.contributor.authorDiaz, Jheimmy
dc.contributor.authorSoza, Andrea
dc.contributor.authorYuseff, Maria Isabel
dc.date.accessioned2025-01-20T22:21:43Z
dc.date.available2025-01-20T22:21:43Z
dc.date.issued2021
dc.description.abstractThe formation of an immune synapse (IS) enables B cells to capture membrane-tethered antigens, where cortical actin cytoskeleton remodeling regulates cell spreading and depletion of F-actin at the centrosome promotes the recruitment of lysosomes to facilitate antigen extraction. How B cells regulate both pools of actin, remains poorly understood. We report here that decreased F-actin at the centrosome and IS relies on the distribution of the proteasome, regulated by Ecm29. Silencing Ecm29 decreases the proteasome pool associated to the centrosome of B cells and shifts its accumulation to the cell cortex and IS. Accordingly, Ecm29-silenced B cells display increased F-actin at the centrosome, impaired centrosome and lysosome repositioning to the IS and defective antigen extraction and presentation. Ecm29-silenced B cells, which accumulate higher levels of proteasome at the cell cortex, display decreased actin retrograde flow in lamellipodia and enhanced spreading responses. Our findings support a model where B the asymmetric distribution of the proteasome, mediated by Ecm29, coordinates actin dynamics at the centrosome and the IS, promoting lysosome recruitment and cell spreading.
dc.fuente.origenWOS
dc.identifier.doi10.3389/fcell.2021.650817
dc.identifier.issn2296-634X
dc.identifier.urihttps://doi.org/10.3389/fcell.2021.650817
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/94658
dc.identifier.wosidWOS:000655051800001
dc.language.isoen
dc.revistaFrontiers in cell and developmental biology
dc.rightsacceso restringido
dc.subjectproteasome
dc.subjectactin cytoskeleton
dc.subjectEcm29
dc.subjectimmune synapse
dc.subjectlysosomes
dc.subjectmicrotubules
dc.subjectB cell
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleEcm29-Dependent Proteasome Localization Regulates Cytoskeleton Remodeling at the Immune Synapse
dc.typeartículo
dc.volumen9
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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