Growth hormone of dried blood spot for the diagnosis of growth hormone deficiencyHormona de crecimiento en sangre de papel filtro para el diagnóstico de deficiencia de hormona de crecimiento

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dc.contributor.authorDomínguez-Menéndez G.
dc.contributor.authorCifuentes L.
dc.contributor.authorLagos M.
dc.contributor.authorQuiroga T.
dc.contributor.authorRumié H.
dc.contributor.authorMartínez-Aguayo A.
dc.contributor.authorLagos M.
dc.contributor.authorQuiroga T.
dc.contributor.authorGonzález C.
dc.contributor.authorRumié H.
dc.contributor.authorMartínez-Aguayo A.
dc.contributor.authorTorres C.
dc.date.accessioned2024-01-10T12:36:56Z
dc.date.available2024-01-10T12:36:56Z
dc.date.issued2019
dc.description.abstract© 2019, Sociedad Chilena de Pediatria. All rights reserved.Introduction: The diagnosis of growth hormone deficiency (GHD) is difficult to determine, and could be associated with severe complications, especially in the neonatal period. The stimulation test of growth hormone (GH) secretion is considered the gold standard for diagnosis, but it has methodological complications and is associated with adverse effects. Neonates present physiological increased secretion of GH, representing a diagnostic window. Objective: To evaluate if the dried blood spot on filter paper obtained in the neonatal period, as part of a neonatal screening for congenital hypothyroidism and phenylketonuria, allows differentiating patients with GHD from those who do not have it. Patients and Method: Study of cases and controls by measuring the GH concentration in dried blood spot on filter paper obtained in the neonatal period, comparing controls with GHD with cases with discarded deficiency. The sample was extracted from the filter paper, obtaining two 0.125 inch discs per each patient from the center of the blood spot on the paper, for a highly sensitive ELISA assay for human GH based on the use of polyclonal antibodies against 22 kDa recombinant human GH. Results: Seven cases of GHD and ten controls were obtained. The median GH concentration of the dried blood spot in the cases is 2.0 ng/ml (Interquartile range 3.6 ng/ml) and 2.05 ng/ml (Interquartile range 2.0 ng/ml) in the controls, Mann-Whitney U test 30.5 (p = 0.68). The two cases with multiple pituitary-hormone deficiency (MPHD) present concentrations lower than 1 ng/ml. Conclusion: The dried blood spot sample did not differentiate GHD patients from control cases, although MPHD cases present much lower concentrations compared to isolated growth hormone deficiency (IGHD).
dc.fechaingreso.objetodigital2024-05-23
dc.fuente.origenScopus
dc.identifier.doi10.32641/rchped.v90i2.674
dc.identifier.eissn07176228
dc.identifier.issn07176228 03704106
dc.identifier.pubmedidMEDLINE:31095230
dc.identifier.scopusidSCOPUS_ID:85065997866
dc.identifier.urihttps://doi.org/10.32641/rchped.v90i2.674
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/76692
dc.identifier.wosidWOS:000465526800004
dc.information.autorucFacultad de Medicina; Martinez Aguayo, Alejandro Gregorio; S/I; 1003862
dc.language.isoes
dc.nota.accesocontenido completo
dc.pagina.final151
dc.pagina.inicio145
dc.publisherSociedad Chilena de Pediatria
dc.revistaRevista Chilena de Pediatria
dc.rightsacceso abierto
dc.subjectDried blood spot testing
dc.subjectDwarfism
dc.subjectGrowth hormone deficiency
dc.subjectPituitary gland
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleGrowth hormone of dried blood spot for the diagnosis of growth hormone deficiencyHormona de crecimiento en sangre de papel filtro para el diagnóstico de deficiencia de hormona de crecimiento
dc.typeartículo
dc.volumen90
sipa.codpersvinculados1003862
sipa.indexWos
sipa.indexScopus
sipa.indexPubmed
sipa.trazabilidadCarga SIPA;09-01-2024
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