Respuesta Transcriptómica de la Variedad de Poroto Palo (Raza Chile) al Estrés Térmico Moderado

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dc.catalogadorgrr
dc.contributor.advisorSchwember Neira, Andrés R.
dc.contributor.authorLecaros Suarez, Barbara
dc.contributor.otherPontificia Universidad Católica de Chile. Facultad de Agronomía y Sistemas Naturales
dc.date.accessioned2025-07-15T19:36:41Z
dc.date.available2025-07-15T19:36:41Z
dc.date.issued2025
dc.date.updated2025-07-15T15:46:56Z
dc.descriptionTesis (Magíster en Fisiología y Producción Vegetal)--Pontificia Universidad Católica de Chile, 2025
dc.description.abstract Common bean (Phaseolus vulgaris L.) is a legume of high nutritional and agronomic value, whose yield is affected by heat conditions associated with climate change. The aim of this study was to evaluate the effect of moderate heat stress applied at the beginning of pod filling on physiological, biochemical, and transcriptomic variables in leaves and seeds of P. vulgaris Chilean race (Palo variety) grown in greenhouse conditions. Reproductive parameters, total protein content, chlorophyll, nitrogen, anthocyanins, and flavonoids were measured. In parallel, gene expression was analyzed using RNA sequencing, differential expression analysis, functional enrichment, and data visualization tools such as principal component analysis, volcano plots, and heatmaps. A 38.2% reduction in total seed weight per plant and a 16.7% decrease in average seed weight were observed, along with transient increases in protein content and changes in chlorophyll and nitrogen levels post-stress. At the transcriptomic level, differentially expressed genes associated with heat response were identified, including members of the HSP20 family and “squamosa-binding-like" transcription factors. A glycine-rich protein gene also stood out, with specific expression in leaves during the post-stress phase, suggesting a potential role in local heat response; although not yet characterized in P. vulgaris, this type of gene has been linked to cell wall remodeling and abiotic stress response in other species. These results reveal a tissue- and stage-specific response to moderate heat stress and provide a foundation for future genetic improvement efforts in local common bean varieties.
dc.fechaingreso.objetodigital2025-07-14
dc.format.extent101 páginas
dc.fuente.origenAutoarchivo
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/104933
dc.information.autorucFacultad de Agronomía e Ingenieria Forestal; Schwember Neira, Andrés R.; 0000-0003-1862-3110; 67919
dc.information.autorucFacultad de Agronomía e Ingenieria Forestal; Lecaros Suarez, Barbara; S/I; 1246340
dc.language.isoes
dc.nota.accesocontenido completo
dc.rightsacceso abierto
dc.rights.licenseAtribución 4.0 Internacional (CC BY 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/deed.es
dc.subjectPhaseolus vulgaris
dc.subjectPalo
dc.subjectRNA-seq
dc.subjectModerate heat stress
dc.subject.ddc600
dc.subject.deweyTecnologíaes_ES
dc.subject.ods02 Zero hunger
dc.subject.ods13 Climate action
dc.subject.odspa02 Hambre cero
dc.subject.odspa13 Acción por el clima
dc.titleRespuesta Transcriptómica de la Variedad de Poroto Palo (Raza Chile) al Estrés Térmico Moderado
dc.typetesis de maestría
sipa.codpersvinculados67919
sipa.codpersvinculados1246340
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3.04 Tesis doctorado