Oxidized phospholipid and transcriptomic signatures of THC-related vaping associated lung injury

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dc.article.number31622
dc.contributor.authorSuber, Tomeka L.
dc.contributor.authorTabary, Mohammadreza
dc.contributor.authorBain, William
dc.contributor.authorOlonisakin, Tolani
dc.contributor.authorLockwood, Karina
dc.contributor.authorXiong, Zeyu
dc.contributor.authorZhang, Yingze
dc.contributor.authorKohli, Naina
dc.contributor.authorFurguiele, Lauren
dc.contributor.authorPeñaloza Cerda, Hernán Felipe
dc.contributor.authorMcverry, Bryan J.
dc.contributor.authorRose, Jason J.
dc.contributor.authorShah, Faraaz
dc.contributor.authorMethe, Barbara
dc.contributor.authorLi, Kelvin
dc.contributor.authorMallampalli, Rama K.
dc.contributor.authorChen, Kong
dc.contributor.authorFan, Li
dc.contributor.authorMorris, Alison
dc.contributor.authorTyurin, Vladimir A.
dc.contributor.authorSamovich, Svetlana N.
dc.contributor.authorBayir, Hulya
dc.contributor.authorTyurina, Yulia Y.
dc.contributor.authorKagan, Valerian
dc.contributor.authorLee, Janet S.
dc.date.accessioned2025-05-15T10:30:02Z
dc.date.available2025-05-15T10:30:02Z
dc.date.issued2024
dc.description.abstractE-cigarette/vaping-associated lung injury (EVALI) is strongly associated with vitamin E acetate and often occurs with concomitant tetrahydrocannabinol (THC) use. To uncover pathways associated with EVALI, we examined cytokines, transcriptomic signatures, and lipidomic profiles in bronchoalveolar lavage fluid (BALF) from THC-EVALI patients. At a single center, we prospectively enrolled mechanically ventilated patients with EVALI from THC-containing products (N = 4) and patients with non-vaping acute lung injury and airway controls (N = 5). BALF samples were analyzed by Luminex multiplex assay, RNA sequencing, and mass spectrometry. After treating BEAS-2B lung epithelial cells with vaping and non-vaping BALF, LDH release was quantified. THC-EVALI BALF had significant increases in IFN gamma, CCL2, CXCL5, and MMP2 relative to non-vaping patients. RNA sequencing showed enrichment for biological oxidation, glucuronidation, and fatty acid metabolism pathways. Oleic acid and arachidonic acid metabolites were increased in THC-EVALI, as were oxidized phosphatidylethanolamines (PE) such as PE(38:4). THC-EVALI BALF induced more LDH release compared to BALF from non-vaping patients. Thus, THC-EVALI is characterized by altered phospholipid composition, accumulation of lipid oxidation products, and increased pro-inflammatory mediators that may contribute to epithelial cell death. These findings serve as a framework to study novel oxidized phospholipids implicated in the pathogenesis of EVALI.
dc.description.funderNational Institutes of Health
dc.format.extent15 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1038/s41598-024-79585-8
dc.identifier.issn2045-2322
dc.identifier.urihttps://doi.org/10.1038/s41598-024-79585-8
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/104254
dc.identifier.wosidWOS:001389341300016
dc.information.autorucFacultad de Ciencias Biológicas; Peñaloza Cerda, Hernán Felipe; S/I; 186994
dc.issue.numero1
dc.language.isoen
dc.nota.accesoContenido parcial
dc.revistaSCIENTIFIC REPORTS
dc.rightsacceso restringido
dc.subjectLipidomics
dc.subjectVaping
dc.subjectAcute lung injury
dc.subjectPhospholipids
dc.subject.ddc610
dc.subject.deweyMedicina y saludes_ES
dc.subject.ods03 Good health and well-being
dc.subject.odspa03 Salud y bienestar
dc.titleOxidized phospholipid and transcriptomic signatures of THC-related vaping associated lung injury
dc.typeartículo
dc.volumen14
sipa.codpersvinculados186994
sipa.indexWOS
sipa.trazabilidadCarga WOS-SCOPUS;15-05-2025
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