Live imaging of developmental processes in a living meristem of <i>Davidia involucrata</i> (Nyssaceae)

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dc.contributor.authorJerominek, Markus
dc.contributor.authorBull-Herenu, Kester
dc.contributor.authorArndt, Melanie
dc.contributor.authorClassen-Bockhoff, Regine
dc.date.accessioned2025-01-23T21:40:35Z
dc.date.available2025-01-23T21:40:35Z
dc.date.issued2014
dc.description.abstractMorphogenesis in plants is usually reconstructed by scanning electron microscopy and histology of meristematic structures. These techniques are destructive and require many samples to obtain a consecutive series of states. Unfortunately, using this methodology the absolute timing of growth and complete relative initiation of organs remain obscure. To overcome this limitation, an in vivo observational method based on Epi-Illumination Light Microscopy (ELM) was developed and tested with a male inflorescence meristem (floral unit) of the handkerchief tree Davidia involucrata Baill. (Nyssaceae). We asked whether the most basal flowers of this floral unit arise in a basipetal sequence or, alternatively, are delayed in their development. The growing meristem was observed for 30 days, the longest live observation of a meristem achieved to date. The sequence of primordium initiation indicates a later initiation of the most basal flowers and not earlier or simultaneously as SEM images could suggest. D. involucrata exemplarily shows that live-ELM gives new insights into developmental processes of plants. In addition to morphogenetic questions such as the transition from vegetative to reproductive meristems or the absolute timing of ontogenetic processes, this method may also help to quantify cellular growth processes in the context of molecular physiology and developmental genetics studies.
dc.fuente.origenWOS
dc.identifier.doi10.3389/fpls.2014.00613
dc.identifier.issn1664-462X
dc.identifier.urihttps://doi.org/10.3389/fpls.2014.00613
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/101625
dc.identifier.wosidWOS:000346993800001
dc.language.isoen
dc.revistaFrontiers in plant science
dc.rightsacceso restringido
dc.subjectlive imaging
dc.subjectin vivo
dc.subjectmorphogenesis
dc.subjectfloral unit meristem (FU meristem)
dc.subjectepi-illumination light microscopy (ELM)
dc.subjectDavidia involucrata
dc.subjectNyssaceae
dc.subject.ods02 Zero Hunger
dc.subject.ods13 Climate Action
dc.subject.odspa02 Hambre cero
dc.subject.odspa13 Acción por el clima
dc.titleLive imaging of developmental processes in a living meristem of <i>Davidia involucrata</i> (Nyssaceae)
dc.typeartículo
dc.volumen5
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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