Inhibition of mouse in vitro fertilization by an antibody against a unique 18-amino acid domain in the polysulfate-binding domain of proacrosin/acrosin

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dc.contributor.authorMoreno, RD
dc.contributor.authorBustamante, E
dc.contributor.authorSchatten, G
dc.contributor.authorBarros, C
dc.date.accessioned2025-01-21T01:30:28Z
dc.date.available2025-01-21T01:30:28Z
dc.date.issued2002
dc.description.abstractObjective: To determine the contribution of the polysulfate-binding domain (PSBD) of acrosin during sperm penetration.
dc.description.abstractDesign: To inhibit the in vitro fertilization of mouse zona-intact oocytes by using a polyclonal antibody raised against an 18-amino acid peptide of proacrosin (anti-PSBD).
dc.description.abstractSetting: Unit of Reproduction and Development, Faculty of Biological Sciences, Pontifical Catholic University of Chile.
dc.description.abstractPatient(s): None.
dc.description.abstractIntervention(s): A polyclonal antibody against the (43)IFMYHNNRRYHTCGGILL(60) peptide was raised in New Zealand female rabbits. The specificity of the antibody was evaluated by an ELISA. Zona-intact mouse oocytes were coincubated with capacitated spermatozoa for 3 hours in the presence of 0.63 mg/mL of the antibody or preimmune serum. As a control, we used zona-free mouse oocytes under the same experimental conditions.
dc.description.abstractMain Outcome Measure(s): We evaluated the fertilization rate of zona-intact and zona-free mouse oocytes by phase-contrast microscopy. An oocyte was considered fertilized when at least one decondensed sperm head was found within the egg cytoplasm. We evaluated 50-60 mouse oocytes in each group in three independent experiments.
dc.description.abstractResult(s): The anti-PSBD antibody inhibited the fertilization of zona-intact, but not zona-free, mouse oocytes, by capacitated spermatozoa. In addition, the binding of the anti-PSBD to proacrosin/acrosin in a solid-phase assay was inhibited in the presence of polysulfates (fucoidan).
dc.description.abstractConclusion(s): The anti-PSBD directed against the PSBD of proacrosin/acrosin inhibited the penetration of capacitated mouse spermatozoa through the zona pellucida. This antibody may be a useful tool to define the roles of the different domains of proacrosin/acrosin during gamete interaction. (C) 2002 by American Society for Reproductive Medicine.
dc.fuente.origenWOS
dc.identifier.issn0015-0282
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/96768
dc.identifier.wosidWOS:000175066400031
dc.issue.numero4
dc.language.isoen
dc.pagina.final817
dc.pagina.inicio812
dc.revistaFertility and sterility
dc.rightsacceso restringido
dc.subjectfertilization
dc.subjectacrosin
dc.subjectacrosome
dc.subjectzona pellucida
dc.subjectsperm
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleInhibition of mouse in vitro fertilization by an antibody against a unique 18-amino acid domain in the polysulfate-binding domain of proacrosin/acrosin
dc.typeartículo
dc.volumen77
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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