ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR RAT AND HUMAN LUTEINIZING-HORMONE

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dc.contributor.authorLEIVA, LA
dc.contributor.authorDELALASTRA, M
dc.date.accessioned2025-01-23T19:22:00Z
dc.date.available2025-01-23T19:22:00Z
dc.date.issued1992
dc.description.abstractA sensitive and specific enzyme-linked immunosorbent assay (ELISA) suitable for measuring rat and human luteinizing hormone (LH) is described. The LH-ELISA used anti-bLH beta subunit monoclonal antibody-coated plates, an antiserum against rLH or hLH and an antibody against rabbit IgG labelled with peroxidase. Using rLH-RP-3 or hLH WHO IRP 68/40 as standard diluted in assay buffer, the LH-ELISA had a sensitivity of 50 pg/ml and 0.78 mIU/ml, respectively. The LH-ELISA allowed accurate determination of LH in buffer, cell culture media, anterior pituitary extracts and sera and was highly specific for rat and human LH. The use of this ELISA offers improvements in convenience, economy, sensitivity and safety over comparable radioimmunoassay procedures.
dc.fuente.origenWOS
dc.identifier.issn0028-3835
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/98934
dc.identifier.wosidWOS:A1992KF09800009
dc.issue.numero5
dc.language.isoen
dc.pagina.final665
dc.pagina.inicio660
dc.revistaNeuroendocrinology
dc.rightsacceso restringido
dc.subjectLUTEINIZING HORMONE
dc.subjectELISA
dc.subjectRAT LH
dc.subjectHUMAN LH
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR RAT AND HUMAN LUTEINIZING-HORMONE
dc.typeartículo
dc.volumen56
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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