Expression and immunolocalization of endothelin peptides and its receptors, ET<sub>A</sub> and ET<sub>B</sub>, in the carotid body exposed to chronic intermittent hypoxia

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dc.contributor.authorRey, Sergio
dc.contributor.authorCorthorn, Jenny
dc.contributor.authorChacon, Cecilia
dc.contributor.authorIturriaga, Rodrigo
dc.date.accessioned2025-01-21T01:05:40Z
dc.date.available2025-01-21T01:05:40Z
dc.date.issued2007
dc.description.abstractIncreased levels of endothelin-1 (ET-1) in the carotid body (CB) contribute to the enhancement of chemosensory responses to acute hypoxia in cats exposed to chronic intermittent hypoxia (CIH). However, it is not known if the ET receptor types A (ETA-R) and B (ETB-R) are upregulated. Thus, we studied the expression and localization of ETA-R and ETB-R using Western blot and immunohistochemistry (IHC) in CBs from cats exposed to cyclic hypoxic episodes, repeated during 8 hr for 4 days. In addition, we determined if ET-1 is expressed in the chemoreceptor cells using double immunofluorescence for ET-1 ancityrosine hydroxylase (TH). We found that ET-1 expression was ubiquitous in the blood vessels and CB parenchyma, although double ET-1 and TH-positive chemoreceptor cells were mostly found in the parenchyma. ETA-R was expressed in most chemoreceptor cells and blood vessels of the CB vascular pole. ETB-R was expressed in chemoreceptor cells, parenchymal capillaries, and blood vessels of the vascular pole. CIH upregulated ETB-R expression by similar to 2.1 (Western blot) and 1.6-fold (IHC) but did not change ETA-R expression. Present results suggest that ET-1, ETA-R, and ETB-R are involved in the enhanced CB chemosensory responses to acute hypoxia induced by CIH.
dc.fuente.origenWOS
dc.identifier.doi10.1369/jhc.6A7079.2006
dc.identifier.eissn1551-5044
dc.identifier.issn0022-1554
dc.identifier.urihttps://doi.org/10.1369/jhc.6A7079.2006
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/95986
dc.identifier.wosidWOS:000243569300007
dc.issue.numero2
dc.language.isoen
dc.pagina.final174
dc.pagina.inicio167
dc.revistaJournal of histochemistry & cytochemistry
dc.rightsacceso restringido
dc.subjectcarotid body
dc.subjectintermittent hypoxia
dc.subjectendothelin receptors
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleExpression and immunolocalization of endothelin peptides and its receptors, ET<sub>A</sub> and ET<sub>B</sub>, in the carotid body exposed to chronic intermittent hypoxia
dc.typeartículo
dc.volumen55
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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