Peroxynitrite oxidizes erythrocyte membrane band 3 protein and diminishes its anion transport capacity

dc.contributor.authorCeledon, Gloria
dc.contributor.authorGonzalez, Gustavo
dc.contributor.authorPino, Jose
dc.contributor.authorLissi, Eduardo A.
dc.date.accessioned2025-01-21T01:05:44Z
dc.date.available2025-01-21T01:05:44Z
dc.date.issued2007
dc.description.abstractWe describe an altered membrane band 3 protein-mediated anion transport in erythrocytes exposed to peroxynitrite, and relate the loss of anion transport to cell damage and to band 3 oxidative modifications. We found that peroxynitrite down-regulate anion transport in a dose dependent relation (100-300 mu moles/1). Hemoglobin oxidation was found at all peroxynitrite concentrations studied. A dose-dependent band 3 protein crosslinking and tyrosine nitration were also observed. Band 3 protein modifications were concomitant with a decrease in transport activity. (-)-Epicatechin avoids band 3 protein nitration but barely affects its transport capacity, suggesting that both processes are unrelated. N-acetyl cysteine partially reverted the loss of band 3 transport capacity. It is concluded that peroxynitrite promotes a decrease in anion transport that is partially due to the reversible oxidation of band 3 cysteine residues. Additionally, band 3 tyrosine nitration seems not to be relevant for the loss of its anion transport capacity.
dc.fuente.origenWOS
dc.identifier.doi10.1080/10715760601090305
dc.identifier.eissn1029-2470
dc.identifier.issn1071-5762
dc.identifier.urihttps://doi.org/10.1080/10715760601090305
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/96003
dc.identifier.wosidWOS:000245588800009
dc.issue.numero3
dc.language.isoen
dc.pagina.final323
dc.pagina.inicio316
dc.revistaFree radical research
dc.rightsacceso restringido
dc.subjecterythrocyte
dc.subjectperoxynitrite
dc.subjectanion transport
dc.subjectband 3
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titlePeroxynitrite oxidizes erythrocyte membrane band 3 protein and diminishes its anion transport capacity
dc.typeartículo
dc.volumen41
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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