Peroxynitrite oxidizes erythrocyte membrane band 3 protein and diminishes its anion transport capacity
dc.contributor.author | Celedon, Gloria | |
dc.contributor.author | Gonzalez, Gustavo | |
dc.contributor.author | Pino, Jose | |
dc.contributor.author | Lissi, Eduardo A. | |
dc.date.accessioned | 2025-01-21T01:05:44Z | |
dc.date.available | 2025-01-21T01:05:44Z | |
dc.date.issued | 2007 | |
dc.description.abstract | We describe an altered membrane band 3 protein-mediated anion transport in erythrocytes exposed to peroxynitrite, and relate the loss of anion transport to cell damage and to band 3 oxidative modifications. We found that peroxynitrite down-regulate anion transport in a dose dependent relation (100-300 mu moles/1). Hemoglobin oxidation was found at all peroxynitrite concentrations studied. A dose-dependent band 3 protein crosslinking and tyrosine nitration were also observed. Band 3 protein modifications were concomitant with a decrease in transport activity. (-)-Epicatechin avoids band 3 protein nitration but barely affects its transport capacity, suggesting that both processes are unrelated. N-acetyl cysteine partially reverted the loss of band 3 transport capacity. It is concluded that peroxynitrite promotes a decrease in anion transport that is partially due to the reversible oxidation of band 3 cysteine residues. Additionally, band 3 tyrosine nitration seems not to be relevant for the loss of its anion transport capacity. | |
dc.fuente.origen | WOS | |
dc.identifier.doi | 10.1080/10715760601090305 | |
dc.identifier.eissn | 1029-2470 | |
dc.identifier.issn | 1071-5762 | |
dc.identifier.uri | https://doi.org/10.1080/10715760601090305 | |
dc.identifier.uri | https://repositorio.uc.cl/handle/11534/96003 | |
dc.identifier.wosid | WOS:000245588800009 | |
dc.issue.numero | 3 | |
dc.language.iso | en | |
dc.pagina.final | 323 | |
dc.pagina.inicio | 316 | |
dc.revista | Free radical research | |
dc.rights | acceso restringido | |
dc.subject | erythrocyte | |
dc.subject | peroxynitrite | |
dc.subject | anion transport | |
dc.subject | band 3 | |
dc.subject.ods | 03 Good Health and Well-being | |
dc.subject.odspa | 03 Salud y bienestar | |
dc.title | Peroxynitrite oxidizes erythrocyte membrane band 3 protein and diminishes its anion transport capacity | |
dc.type | artículo | |
dc.volumen | 41 | |
sipa.index | WOS | |
sipa.trazabilidad | WOS;2025-01-12 |