STUDY OF A PHOTOINDUCED LYSOZYME-RIBOFLAVIN BOND

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dc.contributor.authorFERRER, I
dc.contributor.authorSILVA, E
dc.date.accessioned2025-01-23T19:27:09Z
dc.date.available2025-01-23T19:27:09Z
dc.date.issued1985
dc.description.abstractIrradiation of lysozyme in the presence of riboflavin results in the formation of a lysozyme-riboflavin adduct. Reduction and carboxymethylation of the 4 disulfide bonds as well as the chemical modification of the Tyr residues and the photochemical alteration of the His residue in lysozyme, do not affect the formation of the photo-induced lysozyme-riboflavin bond. When the lysozyme-riboflavin adduct was subjected to mild acid hydrolysis and ion exchange chromatography, the retention of a compound containing 14C-riboflavin was observed. Free 14C-riboflavin, on the contrary, is not retained by the column. The photo-oxidation of free Trp in the presence of 14C-riboflavin, gave a compound which bound to the ion exchange resin like the above-mentioned derivative. The photo-oxidation of the Trp residues in lysozyme and in peptides obtained from lysozyme showed very high quantum yields, and these values were directly related to the incorporation of 14C-riboflavin in these samples.
dc.fuente.origenWOS
dc.identifier.eissn1432-2099
dc.identifier.issn0301-634X
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/99696
dc.identifier.wosidWOS:A1985AHJ1100007
dc.issue.numero1
dc.language.isoen
dc.pagina.final70
dc.pagina.inicio63
dc.revistaRadiation and environmental biophysics
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleSTUDY OF A PHOTOINDUCED LYSOZYME-RIBOFLAVIN BOND
dc.typeartículo
dc.volumen24
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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