Heterologous production of the epoxycarotenoid violaxanthin in <i>Saccharomyces cerevisiae</i>

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dc.contributor.authorCataldo, Vicente F.
dc.contributor.authorArenas, Natalia
dc.contributor.authorSalgado, Valeria
dc.contributor.authorCamilo, Conrado
dc.contributor.authorIbanez, Francisco
dc.contributor.authorAgosin, Eduardo
dc.date.accessioned2025-01-23T19:52:54Z
dc.date.available2025-01-23T19:52:54Z
dc.date.issued2020
dc.description.abstractMicrobial production of carotenoids has mainly focused towards a few products, such as beta-carotene, lycopene and astaxanthin. However, other less explored carotenoids, like violaxanthin, have also shown unique properties and promissory applications. Violaxanthin is a plant-derived epoxidated carotenoid with strong antioxidant activity and a key precursor of valuable compounds, such as fucoxanthin and beta-damascenone. In this study, we report for the first time the heterologous production of epoxycarotenoids in yeast. We engineered the yeast Saccharomyces cerevisiae following multi-level strategies for the efficient accumulation of violaxanthin. Starting from a beta-carotenogenic yeast strain, we first evaluated the performance of several beta-carotene hydroxylases (CrtZ), and zeaxanthin epoxidases (ZEP) from different species, together with their respective N-terminal truncated variants. The combined expression of CrtZ from Pantoea ananatis and truncated ZEP of Haematococcus lacustris showed the best performance and led to a yield of 1.6 mg/g(DCW) of violaxanthin. Further improvement of the epoxidase activity was achieved by promoting the transfer of reducing equivalents to ZEP by expressing several redox partner systems. The co-expression of the plant truncated ferredoxin-3, and truncated root ferredoxin oxidoreductase-1 resulted in a 2.2-fold increase in violaxanthin yield (3.2 mg/g(DCW)). Finally, increasing gene copy number of carotenogenic genes enabled reaching a final production of 7.3 mg/g(DCW) in shake flask cultures and batch bioreactors, which is the highest yield of microbially produced violaxanthin reported to date.
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.ymben.2020.01.006
dc.identifier.eissn1096-7184
dc.identifier.issn1096-7176
dc.identifier.urihttps://doi.org/10.1016/j.ymben.2020.01.006
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/100645
dc.identifier.wosidWOS:000522042900006
dc.language.isoen
dc.pagina.final63
dc.pagina.inicio53
dc.revistaMetabolic engineering
dc.rightsacceso restringido
dc.subjectCarotenoid
dc.subjectXanthophyll
dc.subjectEpoxycarotenoid
dc.subjectViolaxanthin
dc.subjectMetabolic engineering
dc.subjectSaccharomyces cerevisiae
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleHeterologous production of the epoxycarotenoid violaxanthin in <i>Saccharomyces cerevisiae</i>
dc.typeartículo
dc.volumen59
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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