The copy number of the catabolic plasmid pJP4 affects growth of <i>Ralstonia eutropha</i> JMP134 (pJP4) on 3-chlorobenzoate

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dc.contributor.authorTrefault, N
dc.contributor.authorClément, P
dc.contributor.authorManzano, M
dc.contributor.authorPieper, DH
dc.contributor.authorGonzález, B
dc.date.accessioned2025-01-21T01:30:24Z
dc.date.available2025-01-21T01:30:24Z
dc.date.issued2002
dc.description.abstractRalstonia eutropha JMP134 (pJP4) grows on 3-chlorobenzoate (3-CB) or 2,4-dichlorophenoxyacetate (2,4-D). The copy number of chlorocatechol genes has been observed to be important for allowing growth of bacterial strains on chloroaromatic compounds. Despite the fact that two functional chlorocatechol degradation tfd gene clusters are harbored on plasmid pJP4, a single copy of the region comprising all tfd genes in strain JMP134-F was insufficient to allow growth on 3-CB, whereas growth on 2,4-D was only slightly retarded compared to the wild-type strain. Using competitive PCR, approximately five copies of pJP4 per genome were observed to be present in the wild-type strain, whereas only one copy of pJP4 was present per chromosome in strain JMP134-F. Therefore, several copies of pJP4 per chromosome are required for full expression of the tfd-encoded growth abilities in the wild-type R. eutropha strain. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Microbiological Societies.
dc.fuente.origenWOS
dc.identifier.issn0378-1097
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/96739
dc.identifier.wosidWOS:000176618900015
dc.issue.numero1
dc.language.isoen
dc.pagina.final100
dc.pagina.inicio95
dc.revistaFems microbiology letters
dc.rightsacceso restringido
dc.subjectRalstonia eutropha JMP134
dc.subjectpJP4 plasmid
dc.subject3-chlorobenzoate
dc.subjectplasmid copy number
dc.subject2,4-dichlorophenoxyacetate
dc.subjectchromosomal integration
dc.titleThe copy number of the catabolic plasmid pJP4 affects growth of <i>Ralstonia eutropha</i> JMP134 (pJP4) on 3-chlorobenzoate
dc.typeartículo
dc.volumen212
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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