Gold Nanoparticles Mediate Improved Detection of β-amyloid Aggregates by Fluorescence

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dc.contributor.authorJara-Guajardo, Pedro
dc.contributor.authorCabrera, Pablo
dc.contributor.authorCelis, Freddy
dc.contributor.authorSoler, Monica
dc.contributor.authorBerlanga, Isadora
dc.contributor.authorParra-Munoz, Nicole
dc.contributor.authorAcosta, Gerardo
dc.contributor.authorAlbericio, Fernando
dc.contributor.authorGuzman, Fanny
dc.contributor.authorCampos, Marcelo
dc.contributor.authorAlvarez, Alejandra
dc.contributor.authorMorales-Zavala, Francisco
dc.contributor.authorKogan, Marcelo J.
dc.date.accessioned2025-01-23T19:51:29Z
dc.date.available2025-01-23T19:51:29Z
dc.date.issued2020
dc.description.abstractThe early detection of the amyloid beta peptide aggregates involved in Alzheimer's disease is crucial to test new potential treatments. In this research, we improved the detection of amyloid beta peptide aggregates in vitro and ex vivo by fluorescence combining the use of CRANAD-2 and gold nanorods (GNRs) by the surface enhancement fluorescence effect. We synthetized GNRs and modified their surface with HS-PEG-OMe and HS-PEG-COOH and functionalized them with the D1 peptide, which has the capability to selectively bind to amyloid beta peptide. For an in vitro detection of amyloid beta peptide, we co-incubated amyloid beta peptide aggregates with the probe CRANAD-2 and GNR-PEG-D1 observing an increase in the intensity of the fluorescence signal attributed to surface enhancement fluorescence. Furthermore, the surface enhancement fluorescence effect was observed in brain slices of transgenic mice with Alzheimer's disease co-incubated with CRANAD-2 and GNR-PEG-D1. An increase in the fluorescence signal was observed allowing the detection of aggregates that cannot be detected with the single use of CRANAD-2. Gold nanoparticles allowed an improvement in the detection of the amyloid aggregated by fluorescence in vitro and ex vivo.
dc.fuente.origenWOS
dc.identifier.doi10.3390/nano10040690
dc.identifier.eissn2079-4991
dc.identifier.urihttps://doi.org/10.3390/nano10040690
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/100583
dc.identifier.wosidWOS:000539577200093
dc.issue.numero4
dc.language.isoen
dc.revistaNanomaterials
dc.rightsacceso restringido
dc.subjectgold nanorods
dc.subjectCRANAD-2
dc.subjectamyloid beta peptide
dc.subjectSEF
dc.subjectAlzheimer's disease
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleGold Nanoparticles Mediate Improved Detection of β-amyloid Aggregates by Fluorescence
dc.typeartículo
dc.volumen10
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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