Sorting competition with membrane-permeable peptides in intact epithelial cells revealed discrimination of transmembrane proteins not only at the <i>trans</i>-Golgi network but also at pre-Golgi stages

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dc.contributor.authorSoza, A
dc.contributor.authorNorambuena, A
dc.contributor.authorCancino, J
dc.contributor.authorde la Fuente, E
dc.contributor.authorHenklein, P
dc.contributor.authorGonzález, A
dc.date.accessioned2025-01-21T01:08:08Z
dc.date.available2025-01-21T01:08:08Z
dc.date.issued2004
dc.description.abstractTransmembrane proteins destined to the basolateral cell surface of epithelial cells contain in their cytosolic domain at least two classes of sorting signals: one class promotes exit from the endoplasmic reticulum (ER) and transport to the Golgi complex, and the other class operates at the trans-Golgi network (TGN) specifying segregation into basolateral exocytic pathways. Both kinds of addressing motifs are quite diverse among different proteins. It is unclear to what extent this feature reflects alternative decoding mechanisms or variations in motifs recognized by the same sorting factor. Here we applied a novel strategy based on permeable peptide technology and temperature-sensitive model proteins to study competition between cytosolic sorting motifs in the context of mammalian living cells. We used the transduction domain of HIV-1 Tat protein to make a membrane-permeable peptide of the cytosolic tail of GtsO45, which contains a well characterized ER exit di-acidic (DIE) motif and a tyrosine-based basolateral sorting signal (YTDI). This peptide added to the media inhibited transport of GtsO45 from both ER-to-Golgi and TGN-to-basolateral cell surface in transfected Madin-Darby canine kidney cells. Instead, it did not affect the exocytic trafficking of a GtsO45-derived chimeric protein bearing 30 juxtamembrane residues from the cytosolic domain of the epidermal growth factor receptor that contains a variant ER exit motif (ERE) and an unconventional proline-based basolateral sorting signal. These results not only proved the feasibility of competing for sorting events in intact cells but also showed that distinct plasma membrane proteins can be discriminated at pre-TGN stages, and that basolateral sorting involves different recognition elements for tyrosine-based motifs and an unconventional basolateral motif.
dc.fuente.origenWOS
dc.identifier.doi10.1074/jbc.M313197200
dc.identifier.eissn1083-351X
dc.identifier.issn0021-9258
dc.identifier.urihttps://doi.org/10.1074/jbc.M313197200
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/96429
dc.identifier.wosidWOS:000220870400061
dc.issue.numero17
dc.language.isoen
dc.pagina.final17383
dc.pagina.inicio17376
dc.revistaJournal of biological chemistry
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleSorting competition with membrane-permeable peptides in intact epithelial cells revealed discrimination of transmembrane proteins not only at the <i>trans</i>-Golgi network but also at pre-Golgi stages
dc.typeartículo
dc.volumen279
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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