MECHANISM AND KINETIC CHARACTERISTICS OF THE UNCOUPLING BY PLANT STEROIDS OF BILIARY CHOLESTEROL FROM BILE-SALT OUTPUT

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dc.contributor.authorULLOA, N
dc.contributor.authorNERVI, F
dc.date.accessioned2025-01-23T19:27:03Z
dc.date.available2025-01-23T19:27:03Z
dc.date.issued1985
dc.description.abstractIn male Wistar rats fed diets containing different plant steroids, including sitosterols, diosgenin, digitonin and saponin from gypsophila, biliary cholesterol secretion significantly increased 50% to 300%, whereas biliary bile salt and phospholipid showed minor changes. Both cholesterol and phospholipid outputs were coupled to biliary bile salt output in a curvi-linear relationship which could be fitted by rectangular hyperbolae, in the animals fed with different plant steroids. The theoretical maximal biliary cholesterol output significantly increased by 200% in sitosterol-fed rats and 500% in diosgenin-fed animals. No changes were found in the kinetic characteristics of biliary phospholipid outputs. Adding 2% cholesterol to the diosgenin diet abolished the increment of biliary cholesterol output induced by the plant steroid. The intraperitoneal injection of 45 .mu.mol/kg body wt per day(3 days) diosgenin, a C27-sapogenin, and 65 .mu.mol/kg body wt. per day (3 days) tomatidin, a C27-alkaloid, incorporated in phosphatidylcholine-taurocholate liposomes significantly increased biliary cholesterol output by 70%. These experiments indicated that the plant steroid-induced biliary cholesterol output was independent of the inputs of cholesterol from the diet and from hepatic cholesterogenesis modified by the plant steroid. It was apparent that the profound changes of biliary cholesterol secretion were the consequence of direct effects of the steroids on the intrahepatocytic regulatory mechanisms of biliary cholesterol secretion. This novel effect appears to be a universal characteristic of plant steroids, since it can be elicited by sitosterols, C27-sapogenins, C27-alkaloids, and saponins of the cholanic and .beta.-amirinic group.
dc.fuente.origenWOS
dc.identifier.issn0006-3002
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/99684
dc.identifier.wosidWOS:A1985AUQ0800010
dc.issue.numero2
dc.language.isoen
dc.pagina.final189
dc.pagina.inicio181
dc.revistaBiochimica et biophysica acta
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleMECHANISM AND KINETIC CHARACTERISTICS OF THE UNCOUPLING BY PLANT STEROIDS OF BILIARY CHOLESTEROL FROM BILE-SALT OUTPUT
dc.typeartículo
dc.volumen837
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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