EXCITATION OF MESOPHYLL AND BUNDLE SHEATH CHLOROPLASTS OF ATRIPLEX-REPANDA IN THE ABSENCE OF LIGHT

Abstract
Mesophyll and bundle sheath chloroplasts isolated from Atriplex repanda cells promote oxygen consumption by isobutyraldehyde or phenylacetaldehyde. In all cases, a red emission and reduction of tetrazolium blue was observed. Addition of horseradish peroxidase greatly increases the reduction of the dye. In the presence of 3-(3,4-dichlorophenyl)-1,1-dimethylurea, the reduction of the Hill acceptor was fully suppressed. This suppression was abolished when 2,6-dichlorophenolindophenol and ascorbate were added to the systems. These results indicate that, in mesophyll and bundle sheath chloroplasts, chlorophylls can be efficiently excited in the absence of light and an electron flow through the photosystems can be promoted.
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