Optimization of 2D-PAGE protocols for proteomic analysis of two nonaxenic toxin-producing freshwater cyanobacteria: <i>Cylindrospermopsis raciborskii</i> and <i>Raphidiopsis</i> sp.

Simple item page
dc.contributor.authorPlominsky, A. M.
dc.contributor.authorSoto-Liebe, K.
dc.contributor.authorVasquez, M.
dc.date.accessioned2025-01-21T00:08:17Z
dc.date.available2025-01-21T00:08:17Z
dc.date.issued2009
dc.description.abstractAims:
dc.description.abstractTo optimize a protocol for the extraction and an in-depth analysis of the soluble protein fraction of two nonaxenic toxin-producing cyanobacteria Cylindrospermopsis raciborskii (hepatotoxin-producing), and Raphidiopsis sp. (neurotoxin-producing), using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE).
dc.description.abstractMethods and Results:
dc.description.abstractThe soluble protein fractions from strains of C. raciborskii and Raphidiosis sp. with different toxicity phenotypes were analysed by 2D-PAGE. Specific protocols were optimized specifically for each strain. Between 500 and 700 sharp protein spots were distinguished in a single 4-7 pH range 2D-PAGE for each cyanobacterium. Comparison of the protein maps of C. raciborskii CS-505 (a cylindrospermopsin-producing strain) and Raphidiopsis sp. D9 (saxitoxin-producing strain) against the nontoxic C. raciborskii strain CS-509 revealed many unique proteins in each protein map. We confirmed that the resolved proteins were cyanobacterial by identifying three randomly chosen protein spots from a Raphidiopsis sp. strain D9 2D-PAGE, using high-performance liquid chromatography (HPLC) tandem mass spectrometry (MS).
dc.description.abstractConclusions:
dc.description.abstractThe 2D-PAGE conditions presented here provide a robust protocol for proteomic studies in two CYN- and STX-producing model organisms, C. raciborskii and Raphidiopsis sp.
dc.description.abstractSignificance and Impact of the Study:
dc.description.abstractWe present the first protocols for proteomic analyses of Cylindrospermopsis raciborskii and Raphidiopsis sp.
dc.fuente.origenWOS
dc.identifier.doi10.1111/j.1472-765X.2009.02665.x
dc.identifier.eissn1472-765X
dc.identifier.issn0266-8254
dc.identifier.urihttps://doi.org/10.1111/j.1472-765X.2009.02665.x
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/95663
dc.identifier.wosidWOS:000268792600007
dc.issue.numero3
dc.language.isoen
dc.pagina.final337
dc.pagina.inicio332
dc.revistaLetters in applied microbiology
dc.rightsacceso restringido
dc.subject2D-PAGE
dc.subjectcyanobacteria
dc.subjectCylindrospermopsis raciborskii
dc.subjectLC
dc.subjectMS
dc.subjectMS
dc.subjectproteomics
dc.subjectRaphidiopsis sp
dc.subject.ods15 Life on Land
dc.subject.ods06 Clean Water and Sanitation
dc.subject.ods14 Life Below Water
dc.subject.ods13 Climate Action
dc.subject.odspa15 Vida de ecosistemas terrestres
dc.subject.odspa06 Agua limpia y saneamiento
dc.subject.odspa14 Vida submarina
dc.subject.odspa13 Acción por el clima
dc.titleOptimization of 2D-PAGE protocols for proteomic analysis of two nonaxenic toxin-producing freshwater cyanobacteria: <i>Cylindrospermopsis raciborskii</i> and <i>Raphidiopsis</i> sp.
dc.typeartículo
dc.volumen49
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
Files
Collections
Artículos de revistas