Colloidal nanomedicines with prolonged release of chloroquine based on interactions with aromatic polymers after mixing two liquids: from in silico simulation of nanoparticle formation to efficient in-bench scale up

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dc.contributor.authorVillamizar-Sarmiento, Maria Gabriela
dc.contributor.authorYanez, Osvaldo
dc.contributor.authorFlores, Mario E.
dc.contributor.authorAlvarez-Acevedo, Gonzalo
dc.contributor.authorGonzalez-Nilo, Fernando
dc.contributor.authorGuerrero, Juan
dc.contributor.authorMoreno-Villoslada, Ignacio
dc.contributor.authorOyarzun-Ampuero, Felipe A.
dc.date.accessioned2025-01-20T17:09:25Z
dc.date.available2025-01-20T17:09:25Z
dc.date.issued2024
dc.description.abstractNanomedicines containing the aromatic drug chloroquine and the polymer poly(sodium 4-styrenesulfonate) have been theoretically designed and experimentally synthesized following the simple mixture of two aqueous solutions containing the drug and the polymer, respectively. Theoretical calculations show higher binding energy between both the aromatic polymer and chloroquine, and a higher tendency to release water from their hydration spheres, as compared to the binding between the drug and the aliphatic polymer poly(sodium vinyl sulfonate). MD simulations show the spontaneous formation of stable structures of 10 nm of average diameter, even combining short polymer chains, highly diluted reactants, and short reaction time (in the range of mu s). Rapid mixture of the liquids in a stopped flow equipment shows nanoparticle formation in the range of tenths of seconds. Equilibration studies in the range of minutes evidence spheroidal nanoparticles with almost quantitative association efficiency, 48.6 % of drug loading, size of 170 - 410 nm, low polydispersity (PdI = 0.25 - 0.47), and negative zeta potential (-18 - -45 mV). They provide drug release for 30 days, and are stable to NaCl exposure, pH gradient, several temperature values, and long-term storage. Furthermore, we demonstrate scaling up of the nanomedicine production upon increasing the reaction volume. Our studies demonstrate that these highly loaded drug nanoparticles are based on the occurrence of site -specific short-range interactions between the drug and the aromatic excipient such as pi-stacking. In the absence of the aromatic group in the polymer, weak interactions and unstable formulations are evidenced, both theoretically and experimentally. The combination of the selected theoretical and experimental tools could promote the efficient production of drug / polyelectrolyte formulations with therapeutical applications. The chosen components could be considered as potential medicines or as model components to design, develop, characterize, and scale up medicines comprising other combinations of drugs and polymers.
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.molliq.2023.123906
dc.identifier.eissn1873-3166
dc.identifier.issn0167-7322
dc.identifier.urihttps://doi.org/10.1016/j.molliq.2023.123906
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/91040
dc.identifier.wosidWOS:001154489300001
dc.language.isoen
dc.revistaJournal of molecular liquids
dc.rightsacceso restringido
dc.subjectChloroquine
dc.subjectAromatic-aromatic interactions
dc.subjectAromatic polyelectrolytes
dc.subjectNanoparticles
dc.subjectDrug delivery
dc.subjectProlonged release
dc.titleColloidal nanomedicines with prolonged release of chloroquine based on interactions with aromatic polymers after mixing two liquids: from in silico simulation of nanoparticle formation to efficient in-bench scale up
dc.typeartículo
dc.volumen395
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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