Genome analysis and detection of a Chilean isolate of <i>Grapevine leafroll associated virus-3</i>

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dc.contributor.authorEngel, Esteban A.
dc.contributor.authorGirardi, Cristobal
dc.contributor.authorEscobar, Paula F.
dc.contributor.authorArredondo, Vania
dc.contributor.authorDominguez, Calixto
dc.contributor.authorPerez-Acle, Tomas
dc.contributor.authorValenzuela, Pablo D. T.
dc.date.accessioned2025-01-21T01:05:01Z
dc.date.available2025-01-21T01:05:01Z
dc.date.issued2008
dc.description.abstractThe complete genome of the Chilean isolate Cl-766 of Grapevine leafroll-associated virus-3 (GLRaV-3) has been sequenced. This is the first genome sequence obtained from a GLRaV-3 isolate of the Southern hemisphere. The genomic RNA of 17,919 nucleotides contains 13 open reading frames (ORFs) with 5' and 3' untranslated regions (UTR) of 158 and 277 nucleotides, respectively. Comparison with NY1, the only isolate with complete genomic sequence available today, shows 97.6% nucleotide identity between the two isolates. Examination of the genome variability shows that most of the genetic diversity is concentrated in ORF1a. Three additional isolates from different geographic regions of Chile were partially sequenced as well, one which showed sequence divergence with respect to the other local and foreign isolates, indicative of different evolutionary constrains. Immunodetection systems were developed using monoclonal and polyclonal antibodies produced against the recombinant major coat protein of GLRaV-3, providing sensitive and specific detection using a triple antibody sandwich-enzyme linked immunosorbent assay (TAS-ELISA) and an immunocapture-reverse transcription-polymerase chain reaction (IC-RT-PCR) assay.
dc.fuente.origenWOS
dc.identifier.doi10.1007/s11262-008-0241-1
dc.identifier.eissn1572-994X
dc.identifier.issn0920-8569
dc.identifier.urihttps://doi.org/10.1007/s11262-008-0241-1
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/95787
dc.identifier.wosidWOS:000256768300015
dc.issue.numero1
dc.language.isoen
dc.pagina.final118
dc.pagina.inicio110
dc.revistaVirus genes
dc.rightsacceso restringido
dc.subjectClosteroviridae
dc.subjectAmpelovirus
dc.subjectsequencing
dc.subjectELISA
dc.subjectIC-RT-PCR
dc.subject.ods13 Climate Action
dc.subject.ods02 Zero Hunger
dc.subject.odspa13 Acción por el clima
dc.subject.odspa02 Hambre cero
dc.titleGenome analysis and detection of a Chilean isolate of <i>Grapevine leafroll associated virus-3</i>
dc.typeartículo
dc.volumen37
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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