LOCALIZATION OF IMMUNOREACTIVE GLANDULAR KALLIKREIN IN LACTOTROPHS OF THE RAT ANTERIOR-PITUITARY

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dc.contributor.authorVIO, CP
dc.contributor.authorROA, JP
dc.contributor.authorSILVA, R
dc.contributor.authorPOWERS, CA
dc.date.accessioned2025-01-23T19:23:25Z
dc.date.available2025-01-23T19:23:25Z
dc.date.issued1990
dc.description.abstractGlandular kallikrein (a trypsin-like serine protease) is an estrogen-induced and dopamine-repressed protein in the rat anterior pituitary which predominantly exists as a latent zymogen (prokallikrein). Its regulation, presence in estrogen-induced pituitary tumors in F344 rats, and expression in GH3 cells has suggested a localization in lactotrophs (prolactin-producing cells). This study examined the cellular origin of glandular kallikrein using immunocytochemical techniques. Anterior pituitaries from estrogen-treated rats were fixed and embedded in paraffin (for preparation of semi thick sections; 5 .mu.m) or methacrylate (for preparation of thin sections; 1 .mu.m). Glandular kallikrein immunostaining was readily detected in the perinuclear (Golgi) region of parenchymal cells of the anterior pituitary in both thin and semi thick sections. Two-color double immunoperoxidase staining of thin and semi thick sections indicated that glandular kallikrein was localized in cells containing prolactin (PRL) but not other pituitary hormones. Immunoperoxidase staining of consecutive serial thin sections with alterating antisera confirmed a localization of glandular kallikrein in lactotrophs. The results establish that glandular kallikrein is colocalized with PRL in lactotrophs of the rat anterior pituitary. This is consistent with the hypothesis that the function of anterior pituitary glandular kallikrein is linked to PRL in some fashion-possibly as a PRL-processing protease.
dc.fuente.origenWOS
dc.identifier.eissn1423-0194
dc.identifier.issn0028-3835
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/99210
dc.identifier.wosidWOS:A1990CD12600002
dc.issue.numero1
dc.language.isoen
dc.pagina.final+
dc.pagina.inicio10
dc.revistaNeuroendocrinology
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleLOCALIZATION OF IMMUNOREACTIVE GLANDULAR KALLIKREIN IN LACTOTROPHS OF THE RAT ANTERIOR-PITUITARY
dc.typeartículo
dc.volumen51
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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