Advanced glycation endproducts as UVA photosensitizers of tryptophan and ascorbic acid: consequences for the lens

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dc.contributor.authorde La Rochette, A
dc.contributor.authorBirlouez Aragon, L
dc.contributor.authorSilva, E
dc.contributor.authorMorliere, P
dc.date.accessioned2024-01-10T13:10:55Z
dc.date.available2024-01-10T13:10:55Z
dc.date.issued2003
dc.description.abstractUpon aging, the lens accumulates brown fluorophores, mainly derived from the Maillard reaction between vitamin C oxidation products and crystallins lysine residues. At the same time, the concentration of UVA filters decreases, allowing some radiation to be absorbed by lenticular advanced glycation endproducts (AGEs). This paper quantifies the photosensitizing activity of AGEs at various oxygen pressures, and compares it to that of lenticular riboflavin (RF). Solutions containing the sensitizer and the substrates tryptophan (Trp) and ascorbate (AH(-)) were irradiated at 365 nm. We show that the AGEs-photosensitized Tip oxidation rate increases with AGEs concentration and is optimal at 5% oxygen, the pressure in the lens. By contrast, for AH(-), the photooxidation rate increases with oxygen concentration. Despite the higher quantum yield of RF-depending reactions, its low concentration as compared to that of AGEs in aging lenses induces significantly, higher Trp and AH(-) photodegradation rates with AGEs than with RE As ascorbate is more rapidly photodegraded than Trp, the antioxidant competitively protects Trp from oxidation up to I mM, although not absolutely. We conclude that in the aging lens, AH(-) exerts a strong UVA protecting activity, but does not impede some Trp residue to be photodegraded proportionally to the AGEs concentration. (C) 2003 Published by Elsevier Science B.V.
dc.fechaingreso.objetodigital2024-03-17
dc.format.extent7 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1016/S0304-4165(03)00072-2
dc.identifier.issn0304-4165
dc.identifier.pubmedidMEDLINE:12787919
dc.identifier.urihttps://doi.org/10.1016/S0304-4165(03)00072-2
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/77959
dc.identifier.wosidWOS:000183462800001
dc.information.autorucQuímica;Silva E;S/I;98771
dc.issue.numero3
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final241
dc.pagina.inicio235
dc.publisherELSEVIER SCIENCE BV
dc.revistaBIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
dc.rightsacceso restringido
dc.subjectadvanced glycation endproduct
dc.subjectascorbate
dc.subjecttryptophan
dc.subjectUVA
dc.subjectlens
dc.subjectriboflavin
dc.subjectMAILLARD REACTION
dc.subjectMONOCLONAL-ANTIBODIES
dc.subjectOXIDATION-PRODUCTS
dc.subjectSUPEROXIDE ANION
dc.subjectAQUEOUS-HUMOR
dc.subjectVITAMIN-C
dc.subjectL-THREOSE
dc.subjectIN-VITRO
dc.subjectPROTEINS
dc.subjectRIBOFLAVIN
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleAdvanced glycation endproducts as UVA photosensitizers of tryptophan and ascorbic acid: consequences for the lens
dc.typeartículo
dc.volumen1621
sipa.codpersvinculados98771
sipa.indexWOS
sipa.indexScopus
sipa.trazabilidadCarga SIPA;09-01-2024
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