DEVELOPMENT OF HIGHLY SPECIFIC MONOCLONAL-ANTIBODIES FOR THE DIAGNOSIS OF VIBRIO-CHOLERAE-01

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dc.contributor.authorCASTILLO, L
dc.contributor.authorCASTILLO, D
dc.contributor.authorSILVA, W
dc.contributor.authorZAPATA, L
dc.contributor.authorREID, M
dc.contributor.authorULLOA, MT
dc.contributor.authorSEOANE, M
dc.contributor.authorMALDONADO, A
dc.contributor.authorVALENZUELA, ME
dc.contributor.authorBUSTOS, R
dc.contributor.authorTAMPLIN, ML
dc.contributor.authorMARTINEZ, MA
dc.contributor.authorDEIOANNES, AE
dc.contributor.authorJAMETT, A
dc.contributor.authorYUDELEVICH, A
dc.contributor.authorBECKER, MI
dc.date.accessioned2025-01-21T01:34:45Z
dc.date.available2025-01-21T01:34:45Z
dc.date.issued1995
dc.description.abstractWe report here the development of two monoclonal antibodies, termed 5G8 and 5C12, belonging to the IgM and IgG(1) class, respectively, suitable for the identification of Vibrio cholerae 01 in clinical and environmental samples. The specificities of the monoclonals were evaluated by ELISA and indirect immunofluorescent microscopy of microorganisms normally present in stool samples and with two bacterial panels. One panel included 72 potentially antigenically related bacterial strains and the second panel included 20 pathogenic bacterial strains involved in diarrhea cases. The results of these extensive analyses indicate that monoclonal antibodies 5G8 and 5C12 are highly specific and suitable for the clinical diagnosis of Vibrio cholerae 01 inhuman stool samples by indirect immunofluorescent microscopy. Although the antigenic sites recognized by these antibodies were not identified in this study, the observation of Western blot patterns suggested that 5G8 and 5C12 monoclonal antibodies bind to LPS epitopes, a good structural marker for the detection of V. cholerae 01 because it is present in all bacterial cell walls.
dc.fuente.origenWOS
dc.identifier.issn0272-457X
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/97599
dc.identifier.wosidWOS:A1995RF57500010
dc.issue.numero3
dc.language.isoen
dc.pagina.final278
dc.pagina.inicio271
dc.revistaHybridoma
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleDEVELOPMENT OF HIGHLY SPECIFIC MONOCLONAL-ANTIBODIES FOR THE DIAGNOSIS OF VIBRIO-CHOLERAE-01
dc.typeartículo
dc.volumen14
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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