ACTIN-LIKE FILAMENTS IN SYNAPTOSOMES DETECTED BY HEAVY-MEROMYOSIN BINDING
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1976
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Abstract
Cat forebrain synaptosomes were prepared to investigate the presence of actin-like proteins. Actin-like filaments were demonstrated using a heavy meromyosin binding technique. Decorated filaments form networks inside the synaptosomes and were frequently seen in relation to membranes and synaptic vesicles. Using SDS[sodium dodecylsulfate]-polyacrylamide gel electrophoresis, a band corresponding to actin appeared to be one of the major constituents of synaptosomal fractions; this confirmed the work of Fine and coworkers. The significance of actin-like filaments in relation to axoplasmic transport was briefly discussed.